| Title |
Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications
|
|---|---|
| Published in |
AMB Express, April 2017
|
| DOI | 10.1186/s13568-017-0387-0 |
| Pubmed ID | |
| Authors |
Selin Ece, Camilla Lambertz, Rainer Fischer, Ulrich Commandeur |
| Abstract |
Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L(-1) following purification by one-step affinity chromatography. Stability and activity assays using simple lignin model substrates showed that the purified enzyme preparation was active over a broad pH range and at high temperatures, suggesting it would be suitable for biomass degradation. The reusability of ScLac was also demonstrated by immobilizing the enzyme on agarose beads with a binding yield of 33%, and by the synthesis of cross-linked enzyme aggregates with an initial activity recovery of 72%. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 55 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 18% |
| Student > Master | 6 | 11% |
| Researcher | 6 | 11% |
| Professor | 5 | 9% |
| Student > Ph. D. Student | 5 | 9% |
| Other | 4 | 7% |
| Unknown | 19 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 22% |
| Agricultural and Biological Sciences | 8 | 15% |
| Engineering | 5 | 9% |
| Environmental Science | 3 | 5% |
| Immunology and Microbiology | 2 | 4% |
| Other | 4 | 7% |
| Unknown | 21 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 April 2017.
All research outputs
#14,931,166
of 22,965,074 outputs
Outputs from AMB Express
#347
of 1,237 outputs
Outputs of similar age
#183,549
of 309,698 outputs
Outputs of similar age from AMB Express
#19
of 59 outputs
Altmetric has tracked 22,965,074 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,237 research outputs from this source. They receive a mean Attention Score of 2.8. This one has gotten more attention than average, scoring higher than 64% of its peers.
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We're also able to compare this research output to 59 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.