Title |
Molecular characterization of Bacillus thuringiensis using rep-PCR
|
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Published in |
SpringerPlus, November 2013
|
DOI | 10.1186/2193-1801-2-641 |
Pubmed ID | |
Authors |
Rosane Bezerra da Silva, Fernando Hercos Valicente |
Abstract |
The genetic divergence of 65 strains of Bacillus thuringiensis (Bt) was determined using Rep-PCR. Based on the repetitive sequences the BOX primer was the most informative with 26 fragments, followed by ERIC (19) and REP (10), generating a total of 55 fragments. The dendogram shows that ten groups were formed when 45% was the average distance of the population: group 1 with 41,5% of the isolates, 33,8% of the isolates were distributed in other groups and 24,6% did not formed distinct group. 53,2% of the isolates from Embrapa are in the group 1, and 29,8% of the isolates are distributed in other groups. Bt strains from USDA and Institute Pasteur showed more variability. |
X Demographics
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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India | 1 | 2% |
Unknown | 43 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 11 | 25% |
Student > Master | 10 | 23% |
Student > Postgraduate | 5 | 11% |
Researcher | 4 | 9% |
Student > Doctoral Student | 3 | 7% |
Other | 5 | 11% |
Unknown | 6 | 14% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 7 | 16% |
Immunology and Microbiology | 2 | 5% |
Environmental Science | 1 | 2% |
Engineering | 1 | 2% |
Other | 0 | 0% |
Unknown | 8 | 18% |