| Title |
Quantification of calcium content in bone by using ToF-SIMS–a first approach
|
|---|---|
| Published in |
Biointerphases, November 2013
|
| DOI | 10.1186/1559-4106-8-31 |
| Pubmed ID | |
| Authors |
Anja Henss, Marcus Rohnke, Sven Knaack, Matthias Kleine-Boymann, Thomas Leichtweiss, Peter Schmitz, Thaqif El Khassawna, Michael Gelinsky, Christian Heiss, Jürgen Janek |
| Abstract |
The determination of the spatially resolved calcium distribution and concentration in bone is essential for the assessment of bone quality. It enables the diagnosis and elucidation of bone diseases, the course of bone remodelling and the assessment of bone quality at interfaces to implants. With time-of-flight secondary ion mass spectrometry (ToF-SIMS) the calcium distribution in bone cross sections is mapped semi-quantitatively with a lateral resolution of up to 1 μm. As standards for the calibration of the ToF-SIMS data calcium hydroxyapatite collagen scaffolds with different compositions were synthesized. The standards were characterised by loss of ignition, x-ray diffractometry (XRD) and x-ray photoelectron spectroscopy (XPS). The secondary ion count rate for calcium and the calcium content of the standards show a linear dependence. The obtained calibration curve is used for the quantification of the calcium content in the bone of rats. The calcium concentration within an animal model for osteoporosis induction is monitored. Exemplarily the calcium content of the bones was quantified by XPS for validation of the results. Furthermore a calcium mass image is compared with an XPS image to demonstrate the better lateral resolution of ToF-SIMS which advances the locally resolved quantification of the calcium content. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 43% |
| Germany | 1 | 14% |
| United States | 1 | 14% |
| Australia | 1 | 14% |
| Unknown | 1 | 14% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 43% |
| Scientists | 3 | 43% |
| Practitioners (doctors, other healthcare professionals) | 1 | 14% |
Mendeley readers
The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Unknown | 49 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 22% |
| Researcher | 10 | 20% |
| Student > Master | 6 | 12% |
| Student > Bachelor | 3 | 6% |
| Student > Postgraduate | 3 | 6% |
| Other | 7 | 14% |
| Unknown | 10 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 7 | 14% |
| Agricultural and Biological Sciences | 7 | 14% |
| Materials Science | 7 | 14% |
| Medicine and Dentistry | 6 | 12% |
| Biochemistry, Genetics and Molecular Biology | 5 | 10% |
| Other | 7 | 14% |
| Unknown | 11 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2019.
All research outputs
#7,184,094
of 23,942,155 outputs
Outputs from Biointerphases
#109
of 544 outputs
Outputs of similar age
#63,268
of 216,411 outputs
Outputs of similar age from Biointerphases
#1
of 4 outputs
Altmetric has tracked 23,942,155 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 544 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 216,411 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them