| Title |
Diurnal Changes in Transcript and Metabolite Levels during the Iron Deficiency Response of Rice
|
|---|---|
| Published in |
Rice, April 2017
|
| DOI | 10.1186/s12284-017-0152-7 |
| Pubmed ID | |
| Authors |
Jamie Selby-Pham, Adrian Lutz, Laura T. Moreno-Moyano, Berin A. Boughton, Ute Roessner, Alexander A. T. Johnson |
| Abstract |
Rice (Oryza sativa L.) is highly susceptible to iron (Fe) deficiency due to low secretion levels of the mugineic acid (MA) family phytosiderophore (PS) 2'-deoxymugineic acid (DMA) into the rhizosphere. The low levels of DMA secreted by rice have proved challenging to measure and, therefore, the pattern of DMA secretion under Fe deficiency has been less extensively studied relative to other graminaceous monocot species that secrete high levels of PS, such as barley (Hordeum vulgare L.). Gene expression and metabolite analyses were used to characterise diurnal changes occurring during the Fe deficiency response of rice. Iron deficiency inducible genes involved in root DMA biosynthesis and secretion followed a diurnal pattern with peak induction occurring 3-5 h after the onset of light; a result consistent with that of other Strategy II plant species such as barley and wheat. Furthermore, triple quadrupole mass spectrometry identified 3-5 h after the onset of light as peak time of DMA secretion from Fe-deficient rice roots. Metabolite profiling identified accumulation of amines associated with metal chelation, metal translocation and plant oxidative stress responses occurring with peak induction 10-12 h after the onset of light. The results of this study confirmed that rice shares a similar peak time of Fe deficiency associated induction of DMA secretion compared to other Strategy II plant species but has less prominent daily fluctuations of DMA secretion. It also revealed metabolic changes associated with the remediation of Fe deficiency and mitigation of damage from resulting stress in rice roots. This study complements previous studies on the genetic changes in response to Fe deficiency in rice and constitutes an important advance towards our understanding of the molecular mechanisms underlying the rice Fe deficiency response. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 16% |
| Student > Master | 6 | 16% |
| Student > Bachelor | 4 | 11% |
| Researcher | 4 | 11% |
| Student > Doctoral Student | 2 | 5% |
| Other | 4 | 11% |
| Unknown | 11 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 35% |
| Biochemistry, Genetics and Molecular Biology | 4 | 11% |
| Chemistry | 3 | 8% |
| Nursing and Health Professions | 3 | 8% |
| Unspecified | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 12 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 April 2017.
All research outputs
#18,542,806
of 22,965,074 outputs
Outputs from Rice
#251
of 388 outputs
Outputs of similar age
#235,617
of 310,204 outputs
Outputs of similar age from Rice
#7
of 12 outputs
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So far Altmetric has tracked 388 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
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