| Title |
Development of a PCR based marker system for easy identification and classification of aerobic endospore forming bacilli
|
|---|---|
| Published in |
SpringerPlus, November 2013
|
| DOI | 10.1186/2193-1801-2-596 |
| Pubmed ID | |
| Authors |
Sangeeta Kadyan, Manju Panghal, Khushboo Singh, Jaya Parkash Yadav |
| Abstract |
Restriction fragment length analysis of 16S rRNA gene of 52 different aerobic endospore forming Bacilli (AEFB) strains with HaeIII enzyme has revealed the presence of a 460 bp long fragment in 50 AEFB strains. BLAST analysis revealed that the fragment was 463 bp long and it was located at 3' end of 16S rRNA gene. Further specificity of this fragment for AEFB strains was checked by PCR and in silico methods. In PCR based method a primer pair (463 F and 463R) specific to this fragment was designed and this primer pair has shown amplification of 463 bp fragment in AEFB strains only. In in silico methods homology of primer pair and presence of restriction enzyme site in 16S rRNA genes were checked in 268 species of AEFB. Almost all species of AEFB have shown positive results for both of the tests. Further multiple alignments of 463 bp sequences of different species of AEFB have shown that it is a good marker for identification and classification of AEFB. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 3% |
| Unknown | 33 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 21% |
| Researcher | 4 | 12% |
| Lecturer | 3 | 9% |
| Student > Doctoral Student | 3 | 9% |
| Professor | 3 | 9% |
| Other | 12 | 35% |
| Unknown | 2 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 44% |
| Biochemistry, Genetics and Molecular Biology | 6 | 18% |
| Immunology and Microbiology | 3 | 9% |
| Unspecified | 2 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 4 | 12% |
| Unknown | 3 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 November 2013.
All research outputs
#18,353,475
of 22,729,647 outputs
Outputs from SpringerPlus
#1,261
of 1,853 outputs
Outputs of similar age
#159,999
of 215,012 outputs
Outputs of similar age from SpringerPlus
#90
of 102 outputs
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