| Title |
Screening of alginate lyase-excreting microorganisms from the surface of brown algae
|
|---|---|
| Published in |
AMB Express, April 2017
|
| DOI | 10.1186/s13568-017-0361-x |
| Pubmed ID | |
| Authors |
Mingpeng Wang, Lei Chen, Zhaojie Zhang, Xuejiang Wang, Song Qin, Peisheng Yan |
| Abstract |
Alginate lyase is a biocatalyst that degrades alginate to produce oligosaccharides, which have many bioactive functions and could be used as renewable biofuels. Here we report a simple and sensitive plate assay for screening alginate lyase-excreting microorganisms from brown algae. Brown algae Laminaria japonica, Sargassum horneri and Sargassum siliquatrum were cultured in sterile water. Bacteria growing on the surface of seaweeds were identified and their capacity of excreting alginate lyase was analyzed. A total of 196 strains were recovered from the three different algae samples and 12 different bacterial strains were identified capable of excreting alginate lyases. Sequence analysis of the 16S rRNA gene revealed that these alginate lyase-excreting strains belong to eight genera: Paenibacillus (4/12), Bacillus (2/12), Leclercia (1/12), Isoptericola (1/12), Planomicrobium (1/12), Pseudomonas (1/12), Lysinibacillus (1/12) and Sphingomonas (1/12). Further analysis showed that the LJ-3 strain (Bacillus halosaccharovorans) had the highest enzyme activity. To our best knowledge, this is the first report regarding alginate lyase-excreting strains in Paenibacillus, Planomicrobium and Leclercia. We believe that our method used in this study is relatively easy and reliable for large-scale screening of alginate lyase-excreting microorganisms. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 71 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 20% |
| Researcher | 11 | 15% |
| Student > Master | 9 | 13% |
| Student > Bachelor | 8 | 11% |
| Student > Doctoral Student | 3 | 4% |
| Other | 9 | 13% |
| Unknown | 17 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 23% |
| Biochemistry, Genetics and Molecular Biology | 16 | 23% |
| Immunology and Microbiology | 5 | 7% |
| Environmental Science | 4 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
| Other | 9 | 13% |
| Unknown | 19 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 October 2020.
All research outputs
#7,014,019
of 22,962,258 outputs
Outputs from AMB Express
#144
of 1,237 outputs
Outputs of similar age
#111,925
of 308,981 outputs
Outputs of similar age from AMB Express
#10
of 55 outputs
Altmetric has tracked 22,962,258 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 1,237 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 308,981 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.
We're also able to compare this research output to 55 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.