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Comparison of internal process control viruses for detection of food and waterborne viruses

Overview of attention for article published in Applied Microbiology & Biotechnology, March 2017
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15 Mendeley
Title
Comparison of internal process control viruses for detection of food and waterborne viruses
Published in
Applied Microbiology & Biotechnology, March 2017
DOI 10.1007/s00253-017-8244-2
Pubmed ID
Authors

María Dolores Blanco Fernández, Melina Elizabeth Barrios, Robertina Viviana Cammarata, Carolina Torres, Oscar Alberto Taboga, Viviana Andrea Mbayed

Abstract

Enteric viruses are pathogens associated with food- and waterborne outbreaks. The recovery of viruses from food or water samples is affected by the procedures applied to detect and concentrate them. The incorporation of an internal process control virus to the analyses allows monitoring the performance of the methodology. The aim of this study was to produce a recombinant adenovirus (rAdV) and apply it together with bacteriophage PP7 as process controls. The rAdV carries a DNA construction in its genome to differentiate it from wild-type adenovirus by qPCR. The stability of both control viruses was evaluated at different pH conditions. The rAdV was stable at pH 3, 7, and 10 for 18 h. PP7 infectious particles were stable at pH 7 and showed a 2.14 log reduction at pH 10 and total decay at pH 3 after 18 h. Three virus concentration methods were evaluated: hollow-fiber tap water ultrafiltration, wastewater ultracentrifugation, and elution-PEG precipitation from lettuce. Total and infectious viruses were quantified and their recoveries were calculated. Virus recovery for rAdV and PP7 by ultrafiltration showed a wide range (2.10-84.42 and 13.54-84.62%, respectively), whereas that by ultracentrifugation was 5.05-13.71 and 6.98-13.27%, respectively. The performance of ultracentrifugation to concentrate norovirus and enteroviruses present in sewage was not significantly different to the recovery of control viruses. For detection of viruses from lettuce, genomic copies of PP7 were significantly more highly recovered than adenovirus (14.74-18.82 and 0.00-3.44%, respectively). The recovery of infectious virus particles was significantly affected during sewage ultracentrifugation and concentration from lettuce. The simultaneous use of virus controls with dissimilar characteristics and behaviors might resemble different enteric viruses.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 27%
Researcher 2 13%
Unspecified 2 13%
Student > Postgraduate 1 7%
Student > Master 1 7%
Other 5 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 33%
Environmental Science 4 27%
Biochemistry, Genetics and Molecular Biology 3 20%
Unspecified 3 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 January 2018.
All research outputs
#10,622,576
of 13,345,024 outputs
Outputs from Applied Microbiology & Biotechnology
#4,303
of 5,512 outputs
Outputs of similar age
#191,160
of 262,468 outputs
Outputs of similar age from Applied Microbiology & Biotechnology
#38
of 86 outputs
Altmetric has tracked 13,345,024 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,512 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,468 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 86 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.