Title |
Fixation of CO2 in Clostridium cellulovorans analyzed by 13C-isotopomer-based target metabolomics
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Published in |
AMB Express, October 2013
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DOI | 10.1186/2191-0855-3-61 |
Pubmed ID | |
Authors |
Masahiro Shinohara, Hiroshi Sakuragi, Hironobu Morisaka, Hideo Miyake, Yutaka Tamaru, Eiichiro Fukusaki, Kouichi Kuroda, Mitsuyoshi Ueda |
Abstract |
Clostridium cellulovorans has been one of promising microorganisms to use biomass efficiently; however the basic metabolic pathways have not been completely known. We carried out 13C-isotopomer-based target metabolome analysis, or carbohydrate conversion process analysis, for more profound understanding of metabolic pathways of the bacterium. Our findings that pyruvate + oxaloacetate, fumarate, and malate inside and outside cells exhibited 13C incorporation suggest that C. cellulovorans exactly fixed CO2 and partly operated the TCA cycle in a reductive manner. Accompanied with CO2 fixation, the microorganism was also found to produce and secrete lactate. Overall, our study demonstrates that a part of C. cellulovorans metabolic pathways related to glycolysis and the TCA cycle are involved in CO2 fixation. |
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Geographical breakdown
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Unknown | 1 | 50% |
Demographic breakdown
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Mendeley readers
Geographical breakdown
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Researcher | 4 | 13% |
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Professor > Associate Professor | 3 | 10% |
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Unknown | 6 | 20% |
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Nursing and Health Professions | 1 | 3% |
Other | 5 | 17% |
Unknown | 4 | 13% |