| Title |
Fixation of CO2 in Clostridium cellulovorans analyzed by 13C-isotopomer-based target metabolomics
|
|---|---|
| Published in |
AMB Express, October 2013
|
| DOI | 10.1186/2191-0855-3-61 |
| Pubmed ID | |
| Authors |
Masahiro Shinohara, Hiroshi Sakuragi, Hironobu Morisaka, Hideo Miyake, Yutaka Tamaru, Eiichiro Fukusaki, Kouichi Kuroda, Mitsuyoshi Ueda |
| Abstract |
Clostridium cellulovorans has been one of promising microorganisms to use biomass efficiently; however the basic metabolic pathways have not been completely known. We carried out 13C-isotopomer-based target metabolome analysis, or carbohydrate conversion process analysis, for more profound understanding of metabolic pathways of the bacterium. Our findings that pyruvate + oxaloacetate, fumarate, and malate inside and outside cells exhibited 13C incorporation suggest that C. cellulovorans exactly fixed CO2 and partly operated the TCA cycle in a reductive manner. Accompanied with CO2 fixation, the microorganism was also found to produce and secrete lactate. Overall, our study demonstrates that a part of C. cellulovorans metabolic pathways related to glycolysis and the TCA cycle are involved in CO2 fixation. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 3% |
| United States | 1 | 3% |
| Austria | 1 | 3% |
| Unknown | 27 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 5 | 17% |
| Researcher | 4 | 13% |
| Student > Master | 4 | 13% |
| Student > Ph. D. Student | 3 | 10% |
| Professor > Associate Professor | 3 | 10% |
| Other | 5 | 17% |
| Unknown | 6 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 40% |
| Biochemistry, Genetics and Molecular Biology | 4 | 13% |
| Social Sciences | 2 | 7% |
| Engineering | 2 | 7% |
| Nursing and Health Professions | 1 | 3% |
| Other | 5 | 17% |
| Unknown | 4 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2013.
All research outputs
#15,281,593
of 22,725,280 outputs
Outputs from AMB Express
#444
of 1,229 outputs
Outputs of similar age
#129,069
of 209,651 outputs
Outputs of similar age from AMB Express
#4
of 10 outputs
Altmetric has tracked 22,725,280 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,229 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 209,651 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 6 of them.