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TfoI produced by Tepidimonas fonticaldi PL17, a moderate thermophilic bacterium, is an isoschizomer of MseI

Overview of attention for article published in Extremophiles, March 2017
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Title
TfoI produced by Tepidimonas fonticaldi PL17, a moderate thermophilic bacterium, is an isoschizomer of MseI
Published in
Extremophiles, March 2017
DOI 10.1007/s00792-017-0922-6
Pubmed ID
Authors

Ravinder Kumar, Anil Kumar Pinnaka, Beena Krishnan

Abstract

A moderately thermophilic Gram-negative bacterium isolated from the Polok hot spring, Sikkim, India, was identified as a strain (PL17) of Tepidimonas fonticaldi by 16S rDNA sequencing. T. fonticaldi PL17 produces a Type IIP restriction endonuclease; named TfoI. Restriction mapping, run-off sequencing of TfoI-digests of dsDNA fragments, and end compatibility of TfoI with NdeI confirmed that the enzyme recognizes and cleaves the sequence 5'-T^TAA-3', and is thus an isoschizomer of MseI. The TfoI restriction-modification genes in the T. fonticaldi PL17 genome were identified, and the annotated TfoI protein encodes a protein of 181 amino acid residues that shares 47.2% sequence identity with MseI. The native enzyme was purified using a four-column chromatography protocol, and its functional homogeneity was confirmed by standard quality control tests. The ESI-MS measured molecular weight of purified TfoI (20.696 kDa) is in agreement with that of the calculated monomeric molecular weight of the predicted TfoI protein sequence (20.694 kDa). TfoI exhibits optimal activity in the temperature range of 55-70 °C with Mg(+2) or Co(+2) as cofactor. Similar to its isoschizomers, TfoI can be used as the frequent cutter for genome analysis.

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Geographical breakdown

Country Count As %
Unknown 1 100%

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Readers by professional status Count As %
Professor > Associate Professor 1 100%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 100%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 March 2017.
All research outputs
#4,751,113
of 9,227,643 outputs
Outputs from Extremophiles
#239
of 440 outputs
Outputs of similar age
#137,208
of 260,191 outputs
Outputs of similar age from Extremophiles
#8
of 15 outputs
Altmetric has tracked 9,227,643 research outputs across all sources so far. This one is in the 45th percentile – i.e., 45% of other outputs scored the same or lower than it.
So far Altmetric has tracked 440 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
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We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.