| Title |
Isolation of RNA from equine peripheral blood cells: comparison of methods
|
|---|---|
| Published in |
SpringerPlus, September 2013
|
| DOI | 10.1186/2193-1801-2-478 |
| Pubmed ID | |
| Authors |
Zibin Jiang, Cornelius E Uboh, Jinwen Chen, Lawrence R Soma |
| Abstract |
Gene expression studies in equine research involve the use of whole blood samples as a vital source of RNA. To determine the optimal method for RNA isolation from equine whole blood, we compared three RNA isolation strategies using different commercially available kits to evaluate the yield and quality of equine RNA. All 3 methods produced RNA with high quality. Though it did not produce the highest yield, combining the quality, yield and the need for the downstream application in our project, LeukoLOCK™ total RNA isolation system was the best RNA extraction method. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 4% |
| Portugal | 1 | 4% |
| Brazil | 1 | 4% |
| Unknown | 25 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 21% |
| Researcher | 5 | 18% |
| Student > Postgraduate | 3 | 11% |
| Student > Bachelor | 2 | 7% |
| Professor > Associate Professor | 2 | 7% |
| Other | 6 | 21% |
| Unknown | 4 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 29% |
| Biochemistry, Genetics and Molecular Biology | 7 | 25% |
| Veterinary Science and Veterinary Medicine | 4 | 14% |
| Medicine and Dentistry | 2 | 7% |
| Nursing and Health Professions | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 3 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2022.
All research outputs
#4,155,371
of 22,711,242 outputs
Outputs from SpringerPlus
#252
of 1,852 outputs
Outputs of similar age
#37,730
of 202,266 outputs
Outputs of similar age from SpringerPlus
#13
of 105 outputs
Altmetric has tracked 22,711,242 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,852 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 202,266 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 105 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.