| Title |
Characterization of a new oxidant-stable serine protease isolated by functional metagenomics
|
|---|---|
| Published in |
SpringerPlus, August 2013
|
| DOI | 10.1186/2193-1801-2-410 |
| Pubmed ID | |
| Authors |
Sophie Biver, Daniel Portetelle, Micheline Vandenbol |
| Abstract |
A novel serine protease gene, SBcas3.3, was identified by functional screening of a forest-soil metagenomic library on agar plates supplemented with AZCL-casein. Overproduction in Escherichia coli revealed that the enzyme is produced as a 770-amino-acid precursor which is processed to a mature protease of ~55 kDa. The latter was purified by affinity chromatography for characterization with the azocasein substrate. The enzyme proved to be an alkaline protease showing maximal activity between pH 9 and 10 and at 50°C. Treatment with the chelating agent ethylenediaminetetraacetic acid irreversibly denatured the protease, whose stability was found to depend strictly on calcium ions. The enzyme appeared relatively resistant to denaturing and reducing agents, and its activity was enhanced in the presence of 10 ml/l nonionic detergent (Tween 20, Tween 80, or Triton X-100). Moreover, SBcas3.3 displayed oxidant stability, a feature particularly sought in the detergent and bleaching industries. SBcas3.3 was activated by hydrogen peroxide at concentrations up to 10 g/l and it still retained 30% of activity in 50 g/l H2O2. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 3 | 4% |
| Belgium | 1 | 1% |
| Unknown | 75 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 25% |
| Student > Master | 17 | 22% |
| Researcher | 8 | 10% |
| Student > Bachelor | 7 | 9% |
| Student > Doctoral Student | 7 | 9% |
| Other | 12 | 15% |
| Unknown | 8 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 36 | 46% |
| Biochemistry, Genetics and Molecular Biology | 21 | 27% |
| Immunology and Microbiology | 3 | 4% |
| Environmental Science | 2 | 3% |
| Computer Science | 1 | 1% |
| Other | 3 | 4% |
| Unknown | 13 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 September 2013.
All research outputs
#15,279,577
of 22,721,584 outputs
Outputs from SpringerPlus
#932
of 1,852 outputs
Outputs of similar age
#123,594
of 200,084 outputs
Outputs of similar age from SpringerPlus
#46
of 89 outputs
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