| Title |
pPCV, a versatile vector for cloning PCR products
|
|---|---|
| Published in |
SpringerPlus, September 2013
|
| DOI | 10.1186/2193-1801-2-441 |
| Pubmed ID | |
| Authors |
Christiane R Janner, Ana Lívia P Brito, Lidia Maria P Moraes, Viviane CB Reis, Fernando AG Torres |
| Abstract |
The efficiency of PCR product cloning depends on the nature of the DNA polymerase employed because amplicons may have blunt-ends or 3' adenosines overhangs. Therefore, for amplicon cloning, available commercial vectors are either blunt-ended or have a single 3' overhanging thymidine. The aim of this work was to offer in a single vector the ability to clone both types of PCR products. For that purpose, a minimal polylinker was designed to include restriction sites for EcoRV and XcmI which enable direct cloning of amplicons bearing blunt-ends or A-overhangs, respectively, still offering blue/white selection. When tested, the resulting vector, pPCV, presented high efficiency cloning of both types of amplicons. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 43% |
| Student > Ph. D. Student | 2 | 14% |
| Researcher | 2 | 14% |
| Student > Bachelor | 1 | 7% |
| Professor > Associate Professor | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 2 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 50% |
| Biochemistry, Genetics and Molecular Biology | 4 | 29% |
| Immunology and Microbiology | 1 | 7% |
| Unknown | 2 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 September 2013.
All research outputs
#15,279,577
of 22,721,584 outputs
Outputs from SpringerPlus
#932
of 1,852 outputs
Outputs of similar age
#121,002
of 196,918 outputs
Outputs of similar age from SpringerPlus
#47
of 102 outputs
Altmetric has tracked 22,721,584 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,852 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
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We're also able to compare this research output to 102 others from the same source and published within six weeks on either side of this one. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.