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The activation of the IFNβ induction/signaling pathway in porcine alveolar macrophages by porcine reproductive and respiratory syndrome virus is variable

Overview of attention for article published in Veterinary Research Communications, November 2016
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Title
The activation of the IFNβ induction/signaling pathway in porcine alveolar macrophages by porcine reproductive and respiratory syndrome virus is variable
Published in
Veterinary Research Communications, November 2016
DOI 10.1007/s11259-016-9665-6
Pubmed ID
Authors

Christopher C. Overend, Junru Cui, Marvin J. Grubman, Antonio E. Garmendia

Abstract

It has been recognized that the expression of type I interferon (IFNα/β) may be suppressed during infection with porcine reproductive, respiratory syndrome virus (PRRSV). This causes profound negative effects on both the innate and adaptive immunity of the host resulting in persistence of infection. Test the effects of PRRSV infection of porcine alveolar macrophages (PAMs), the main target cell, on the expression of interferon beta (IFNβ) and downstream signaling events. In order to examine those effects, PAMs harvested from lungs of healthy PRRSV-free animals were infected with virulent, attenuated, infectious clone-derived chimeric viruses, or field PRRS virus strains. Culture supernatants from the infected PAMs were tested for IFNβ protein expression by means of indirect ELISA and for bioactivity by a vesicular stomatitis virus plaque reduction assay. The expression of the Mx protein was assayed to ascertain signaling events. These experiments demonstrated that PRRSV does induce variably, the expression of bioactive IFNβ protein in the natural host cell. To further elucidate the effects of PRRSV infection on IFNβ signaling, Mx-1 an interferon stimulated gene (ISG), was also tested for expression. Interestingly, Mx-1 expression by infected PAMs generally correlated with IFNβ production. The results of this study demonstrate that the induction of IFNβ and signaling in PAMs after PRRSV infection is variable.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 20%
Student > Bachelor 1 20%
Student > Ph. D. Student 1 20%
Researcher 1 20%
Student > Doctoral Student 1 20%
Other 0 0%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 1 20%
Unspecified 1 20%
Linguistics 1 20%
Agricultural and Biological Sciences 1 20%
Medicine and Dentistry 1 20%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2017.
All research outputs
#7,909,100
of 9,122,902 outputs
Outputs from Veterinary Research Communications
#123
of 176 outputs
Outputs of similar age
#214,646
of 253,759 outputs
Outputs of similar age from Veterinary Research Communications
#7
of 8 outputs
Altmetric has tracked 9,122,902 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 176 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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