| Title |
Heterogeneous expression of DnaK gene from Alicyclobacillus acidoterrestris improves the resistance of Escherichia coli against heat and acid stress
|
|---|---|
| Published in |
AMB Express, February 2017
|
| DOI | 10.1186/s13568-017-0337-x |
| Pubmed ID | |
| Authors |
Xixi Xu, Lingxia Jiao, Xin Feng, Junjian Ran, Xinhong Liang, Ruixiang Zhao |
| Abstract |
Alicyclobacillus acidoterrestris, an acidophilic and thermophilic bacteria, is an important microbial resource for stress resistance genes screening. In this study, DnaK gene from A. acidoterrestris was subcloned to construct the recombinant plasmid pET28a-DnaK. The successful construction of the plasmid was verified by double-enzyme digestion and sequencing analysis. The recombinant plasmid was transformed into Escherichia coli BL21 and isopropy-β-D-thiogalactoside (IPTG) was used to induce recombinant E. coli to express DnaK gene. A 70 kD fusion protein was identified by SDS-PAGE, which suggested that DnaK gene from A. acidoterrestris was successfully expressed. The recombinant and wild BL21 were treated with high temperatures of 54, 56 and 58 °C at pH values of 5.0-7.0 to compare the effects of heterogeneous expression of the DnaK gene from A. acidoterrestris on the stress resistance. The experimental results showed that survival rate of recombinant BL21-DnaK has been improved considerably under heat and acid stresses in contrast with the wild BL21, and D-values of recombinant BL21 were 14.7-72% higher than that of wild BL21, which demonstrated that heterogeneous expression of DnaK gene from A. acidoterrestris could significantly enhance the resistance of host bacteria E. coli against heat and acid stresses. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Kazakhstan | 1 | 4% |
| Unknown | 25 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 23% |
| Student > Bachelor | 5 | 19% |
| Researcher | 3 | 12% |
| Student > Ph. D. Student | 3 | 12% |
| Professor | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 6 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 27% |
| Biochemistry, Genetics and Molecular Biology | 6 | 23% |
| Immunology and Microbiology | 2 | 8% |
| Nursing and Health Professions | 1 | 4% |
| Chemical Engineering | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 7 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 February 2017.
All research outputs
#15,443,875
of 22,953,506 outputs
Outputs from AMB Express
#445
of 1,237 outputs
Outputs of similar age
#260,256
of 426,820 outputs
Outputs of similar age from AMB Express
#25
of 64 outputs
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