Title |
Proteolytic enzymes from Bromelia antiacantha as tools for controlled tissue hydrolysis in entomology
|
---|---|
Published in |
SpringerPlus, July 2013
|
DOI | 10.1186/2193-1801-2-307 |
Pubmed ID | |
Authors |
Laura Macció, Diego Vallés, Ana Maria Cantera |
Abstract |
A crude extract with high proteolytic activity (78.1 EU/mL), prepared from ripe fruit of Bromelia antiacantha was used to hydrolyze and remove soft tissues from the epigyne of Apopyllus iheringi. This enzymatic extract presented four actives isoforms which have a broad substrate specificity action. Enzyme action on samples was optimized after evaluation under different conditions of pH, enzyme-substrate ratio and time (parameters selected based on previous studies) of treatment (pH 4.0, 6.0 and 8.0 at 42°C with different amount of enzyme). Scanning electron microscopy was used to evaluate conditions resulting in complete digestion of epigyne soft tissues. Optimal conditions for soft tissue removal were 15.6 total enzyme units, pH 6.0 for 18 h at 42°C. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 3 | 38% |
Student > Ph. D. Student | 1 | 13% |
Student > Master | 1 | 13% |
Researcher | 1 | 13% |
Professor > Associate Professor | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 50% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Environmental Science | 1 | 13% |
Unknown | 2 | 25% |