| Title |
Assessment of human telomeric G-quadruplex structures using surface-enhanced Raman spectroscopy
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, January 2017
|
| DOI | 10.1007/s00216-016-0172-y |
| Pubmed ID | |
| Authors |
Snežana Miljanić, Marina Ratkaj, Marija Matković, Ivo Piantanida, Paola Gratteri, Carla Bazzicalupi |
| Abstract |
G-Quadruplex (G4) structures of a human telomeric 24-mer (5'-TTAGGGTTAGGGTTAGGGTTAGGG-3') sequence (Tel24) stabilized by sodium and potassium ions have been assessed using surface-enhanced Raman scattering (SERS) spectroscopy. The distinctive SERS spectra of Tel24 in the presence of 100 mM Na(+) and 100 mM K(+) were obtained and the SERS bands characteristic of the antiparallel basket-type and the mixed hybrid (3+1) structures, respectively, were identified and assigned. The influence of the SERS - active substrate on the scattering enhancement was studied using citrate- and chloride-covered silver nanoparticles, in the absence and presence of the aggregating agent (0.1 M Na2SO4 and 0.1 M K2SO4). The highly reproducible SERS spectra of Tel24 obtained in various SERS active media indicated the same adsorption mechanism of the cation - stabilized G-quadruplexes onto the metal surface, regardless of the silver colloid. The remarkable resemblance between the circular dichroism (CD) spectra of the Tel24 structures with and without the colloid confirmed that interaction with the enhancing silver surface did not affect the stability of the formed G4 structures. The presented study pointed to a great potential of the SERS spectroscopy for the sensitive structural analysis of various G4 topologies. Graphical Abstract SERS spectroscopy allowed identification of Na(+) stabilized antiparallel basket-type and K(+) stabilized hybrid (3+1) structures of the same 24-mer human telomeric sequence. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 32% |
| Student > Ph. D. Student | 3 | 11% |
| Student > Bachelor | 2 | 7% |
| Professor > Associate Professor | 2 | 7% |
| Student > Master | 2 | 7% |
| Other | 4 | 14% |
| Unknown | 6 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 13 | 46% |
| Biochemistry, Genetics and Molecular Biology | 3 | 11% |
| Medicine and Dentistry | 3 | 11% |
| Agricultural and Biological Sciences | 1 | 4% |
| Materials Science | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 7 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2017.
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#20,656,161
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Outputs from Analytical & Bioanalytical Chemistry
#6,601
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#320,372
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#76
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