| Title |
Characterisation of enterovirus 71 replication kinetics in human colorectal cell line, HT29
|
|---|---|
| Published in |
SpringerPlus, June 2013
|
| DOI | 10.1186/2193-1801-2-267 |
| Pubmed ID | |
| Authors |
Yan Long Edmund Lui, Peter Timms, Louise Marie Hafner, Tuan Lin Tan, Kian Hwa Tan, Eng Lee Tan |
| Abstract |
Hand, Foot and Mouth Disease (HFMD), a contagious viral disease that commonly affects infants and children with blisters and flu like symptoms, is caused by a group of enteroviruses such as Enterovirus 71 (EV71) and coxsackievirus A16 (CA16). However some HFMD caused by EV71 may further develop into severe neurological complications such as encephalitis and meningitis. The route of transmission was postulated that the virus transmit from one person to another through direct contact of vesicular fluid or droplet from the infected or via faecal-oral route. To this end, this study utilised a human colorectal adenocarcinoma cell line (HT29) with epithelioid morphology as an in vitro model for the investigation of EV71 replication kinetics. Using qPCR, viral RNA was first detected in HT29 cells as early as 12 h post infection (hpi) while viral protein was first detected at 48 hpi. A significant change in HT29 cells' morphology was also observed after 48 hpi. Furthermore HT29 cell viability also significantly decreased at 72 hpi. Together, data from this study demonstrated that co-culture of HT29 with EV71 is a useful in vitro model to study the pathogenesis of EV71. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 29% |
| Researcher | 3 | 21% |
| Student > Postgraduate | 2 | 14% |
| Student > Master | 2 | 14% |
| Student > Bachelor | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 21% |
| Medicine and Dentistry | 3 | 21% |
| Agricultural and Biological Sciences | 3 | 21% |
| Veterinary Science and Veterinary Medicine | 1 | 7% |
| Mathematics | 1 | 7% |
| Other | 2 | 14% |
| Unknown | 1 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 June 2013.
All research outputs
#18,340,605
of 22,712,476 outputs
Outputs from SpringerPlus
#1,260
of 1,852 outputs
Outputs of similar age
#147,794
of 196,784 outputs
Outputs of similar age from SpringerPlus
#64
of 76 outputs
Altmetric has tracked 22,712,476 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,852 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
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We're also able to compare this research output to 76 others from the same source and published within six weeks on either side of this one. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.