| Title |
Molecular cloning, polymorphism, and functional activity of the bovine and water buffalo Mx2 gene promoter region
|
|---|---|
| Published in |
SpringerPlus, December 2016
|
| DOI | 10.1186/s40064-016-3729-5 |
| Pubmed ID | |
| Authors |
H. A. E. Babiker, T. Saito, Y. Nakatsu, S. Takasuga, M. Morita, Y. Sugimoto, J. Ueda, T. Watanabe |
| Abstract |
Bovine Mx2 gene sequences were already reported, but further information about the gene properties is not yet available. The objective of the current study was to elucidate the structural properties of the bovine Mx2 gene mainly the promoter region and its possible functional role. If available, such information would help in assessing the functional properties of the gene, which was reported to confer antiviral action against recombinant VSV. Examinations on the bovine genomic BAC clone-confirmed to contain the Mx2 gene-revealed 883-bp sequences. A computer scan unequivocally identified a 788-bp promoter region containing a typical TATA box, three ISREs and other promoter-specific motifs. Comparative analysis of nine bovine genomic DNA samples showed 19 nucleotide substitutions suggesting the existence of five different genotypes in the promoter region. The water buffalo Mx2 promoter region was determined by using primers based on the bovine Mx2 promoter region disclosing 893-bp, with 56 substitutions, two insertions, 9 and 1 nt at two different sites. A functional analysis of the putative ISRE indicated that ISRE played a synergetic role in the activation of bovine Mx2 gene transcription. Bovine and water buffalo Mx2 promoter region was identified disclosing, the conserved ISRE, located in the proximal end of the promoter region like other members of the antiviral family, suggesting functional activity under interferon stimulation. |
X Demographics
The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 33% |
| United Kingdom | 2 | 22% |
| Unknown | 4 | 44% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 78% |
| Scientists | 1 | 11% |
| Practitioners (doctors, other healthcare professionals) | 1 | 11% |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 18% |
| Student > Bachelor | 2 | 18% |
| Unspecified | 1 | 9% |
| Other | 1 | 9% |
| Professor | 1 | 9% |
| Other | 2 | 18% |
| Unknown | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 36% |
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Unspecified | 1 | 9% |
| Computer Science | 1 | 9% |
| Engineering | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 2 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 December 2016.
All research outputs
#5,219,991
of 24,995,564 outputs
Outputs from SpringerPlus
#311
of 1,867 outputs
Outputs of similar age
#97,528
of 432,253 outputs
Outputs of similar age from SpringerPlus
#11
of 43 outputs
Altmetric has tracked 24,995,564 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,867 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one has done well, scoring higher than 83% of its peers.
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We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.