| Title |
Bioequivalance and pharmacokinetic study of febuxostat in human plasma by using LC-MS/MS with liquid liquid extraction method
|
|---|---|
| Published in |
SpringerPlus, April 2013
|
| DOI | 10.1186/2193-1801-2-194 |
| Pubmed ID | |
| Authors |
Babu Rao Chandu, Kanchanamala Kanala, Nagiat T Hwisa, Prakash Katakam, Mukkanti Khagga |
| Abstract |
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human plasma using Febuxostat D7 (FBD7) as an internal standard (IS) was used. Chromatographic separation was performed on Ascentis Express C18 (50x4.6 mm, 3.5 μ) column. Mobile phase composed of 10 mM Ammonium formate: Acetonitrile (20:80 v/v), with 0.8 mL/min flow-rate. Drug and IS were extracted by Liquid- liquid extraction. FB and FBD7 were detected with proton adducts at m/z 317.1→261.1 and 324.2→262.1 in multiple reaction monitoring (MRM) positive mode respectively. The method was validated with the correlation coefficients of (r(2)) ≥ 0.9850 over a linear concentration range of 1.00-8000.00 ng/mL. This method demonstrated intra and inter-day precision within 2.64 to 3.88 and 2.76 to 8.44% and accuracy within 97.33 to 99.05 and 100.30 to 103.19% for FB. This method is successfully applied in the Bioequivalence study of 9 human volunteers. |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 25% |
| Student > Bachelor | 4 | 17% |
| Student > Postgraduate | 4 | 17% |
| Student > Ph. D. Student | 3 | 13% |
| Researcher | 2 | 8% |
| Other | 5 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 9 | 38% |
| Medicine and Dentistry | 5 | 21% |
| Chemistry | 4 | 17% |
| Agricultural and Biological Sciences | 3 | 13% |
| Psychology | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 1 | 4% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 April 2013.
All research outputs
#20,191,579
of 22,708,120 outputs
Outputs from SpringerPlus
#1,461
of 1,852 outputs
Outputs of similar age
#167,408
of 192,344 outputs
Outputs of similar age from SpringerPlus
#57
of 103 outputs
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