| Title |
Assay of hemoglobin A1c using lectin from Aleuria aurantia
|
|---|---|
| Published in |
AMB Express, November 2016
|
| DOI | 10.1186/s13568-016-0288-7 |
| Pubmed ID | |
| Authors |
Masato Kabata, Erina Hase, Kouta Kimura, Yuka Kobayashi, Yasushi Ueno, Kazuaki Yoshimune |
| Abstract |
Hemoglobin A1c (HbA1c) has an N-terminal fructosyl valine on the β-chain, and this modification is caused by the non-enzymatic glycosylation of hemoglobin (Hb). The relative concentration ratio of HbA1c to total Hb is an important biomarker for the diagnosis of diabetes. HbA1c-binding lectins were screened from 29 sources of lectin, and the lectin from Aleuria aurantia (AAL) was revealed to have higher affinity to HbA1c than to Hb. The concentration of HbA1c was determined by lectin-based enzyme-linked immunosorbent assay (ELISA) using the AAL lectin. Higher reproducibility of the assay was observed at 4 °C than at 25 and 37 °C. This observation is consistent with the known temperature-dependent behavior of lectins. Preincubation of HbA1c with an anti-HbA1c antibody inhibited the binding, suggesting that AAL binds to the N-terminal fructosyl valine epitope of HbA1c. Higher inhibitory effect was observed for 10 mM D-fructose than for the same concentrations of L-fucose, D-fucose, or D-glucose. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 50% |
| Professor | 1 | 17% |
| Professor > Associate Professor | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 33% |
| Nursing and Health Professions | 1 | 17% |
| Agricultural and Biological Sciences | 1 | 17% |
| Neuroscience | 1 | 17% |
| Unknown | 1 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 November 2016.
All research outputs
#15,395,259
of 22,903,988 outputs
Outputs from AMB Express
#446
of 1,236 outputs
Outputs of similar age
#249,594
of 415,136 outputs
Outputs of similar age from AMB Express
#19
of 44 outputs
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