I wonder if an annular ring of light (like in phase contrast) instead of brightfield is used to create the images for cross-correlation. Would the phase contrast images allow for a larger change of image wrt change in Z position, which allows for better dr
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@christlet If anyone else is going down the rabbit hole of finding fiducial-free axial drift correction methods, the 3D drift correction method using brightfield imaging from Bo Huang's lab (https://t.co/GQxSd2e1aJ) obtained about +/- 30 nm (FWHM) of Z sta
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@HohlbeinLab @ewers_helge @seamus_holden Without giving anything away that is not already published, it seems to be a particularly well executed version of the McGorty et al approach https://t.co/vEUw3mvERO with a couple of small wrinkles to make it perfor