| Title |
Intergeneric fusant development using chitinase preparation of Rhizopus stolonifer NCIM 880
|
|---|---|
| Published in |
AMB Express, November 2016
|
| DOI | 10.1186/s13568-016-0287-8 |
| Pubmed ID | |
| Authors |
Kailas D. Sonawane, Narayan R. Dandagal, Akibjaved G. Naikwadi, Piyush T. Gurav, Samar V. Anapat, Naiem H. Nadaf, Deepak B. Jadhav, Shailesh R. Waghmare |
| Abstract |
Fungal chitinase have tremendous applications in biotech industries, with this approach we focused on extracellular chitinase from Rhizopus stolonifer NCIM 880 for the formation of fungal protoplasts. The maximum chitinase production reached after 24 h at 2.5% colloidal chitin concentration in presence of starch as an inducer. Chitinase was extracted efficiently at 65% cold acetone concentration and then purified by using DEAE-Cellulose column chromatography. Purified chitinase having molecular weight 22 kDa with single polypeptide chain was optimally active at pH 5.0 and temperature 30 °C. The purified chitinase revealed kinetic properties like Km 1.66 mg/ml and Vmax 769 mM/min. Crude chitinase extract efficiently formed protoplasts from A. niger, A. oryzae, T. viride and F. moniliforme. The formed protoplasts of A. niger and T. viride showed 70 and 66% regeneration frequency respectively. Further, intergeneric fusants were developed successfully and identified at molecular level using RNA profiling. Thus, this study could be useful for strain improvement of various fungi for biotechnological applications. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 24% |
| Student > Bachelor | 5 | 24% |
| Lecturer > Senior Lecturer | 2 | 10% |
| Student > Master | 2 | 10% |
| Other | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 4 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 48% |
| Agricultural and Biological Sciences | 3 | 14% |
| Immunology and Microbiology | 2 | 10% |
| Economics, Econometrics and Finance | 1 | 5% |
| Engineering | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 4 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 November 2016.
All research outputs
#15,393,913
of 22,901,818 outputs
Outputs from AMB Express
#446
of 1,236 outputs
Outputs of similar age
#194,051
of 307,483 outputs
Outputs of similar age from AMB Express
#17
of 46 outputs
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