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Intergeneric fusant development using chitinase preparation of Rhizopus stolonifer NCIM 880

Overview of attention for article published in AMB Express, November 2016
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16 Mendeley
Title
Intergeneric fusant development using chitinase preparation of Rhizopus stolonifer NCIM 880
Published in
AMB Express, November 2016
DOI 10.1186/s13568-016-0287-8
Pubmed ID
Authors

Kailas D. Sonawane, Kailas D. Sonawane, Narayan R. Dandagal, Akibjaved G. Naikwadi, Piyush T. Gurav, Samar V. Anapat, Naiem H. Nadaf, Deepak B. Jadhav, Shailesh R. Waghmare

Abstract

Fungal chitinase have tremendous applications in biotech industries, with this approach we focused on extracellular chitinase from Rhizopus stolonifer NCIM 880 for the formation of fungal protoplasts. The maximum chitinase production reached after 24 h at 2.5% colloidal chitin concentration in presence of starch as an inducer. Chitinase was extracted efficiently at 65% cold acetone concentration and then purified by using DEAE-Cellulose column chromatography. Purified chitinase having molecular weight 22 kDa with single polypeptide chain was optimally active at pH 5.0 and temperature 30 °C. The purified chitinase revealed kinetic properties like Km 1.66 mg/ml and Vmax 769 mM/min. Crude chitinase extract efficiently formed protoplasts from A. niger, A. oryzae, T. viride and F. moniliforme. The formed protoplasts of A. niger and T. viride showed 70 and 66% regeneration frequency respectively. Further, intergeneric fusants were developed successfully and identified at molecular level using RNA profiling. Thus, this study could be useful for strain improvement of various fungi for biotechnological applications.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 25%
Researcher 3 19%
Lecturer > Senior Lecturer 2 13%
Student > Master 2 13%
Professor 1 6%
Other 2 13%
Unknown 2 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 50%
Agricultural and Biological Sciences 3 19%
Immunology and Microbiology 2 13%
Economics, Econometrics and Finance 1 6%
Unknown 2 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 November 2016.
All research outputs
#4,651,997
of 8,629,768 outputs
Outputs from AMB Express
#235
of 532 outputs
Outputs of similar age
#133,611
of 240,761 outputs
Outputs of similar age from AMB Express
#16
of 52 outputs
Altmetric has tracked 8,629,768 research outputs across all sources so far. This one is in the 27th percentile – i.e., 27% of other outputs scored the same or lower than it.
So far Altmetric has tracked 532 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 240,761 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.