| Title |
Bacillus megaterium—from simple soil bacterium to industrial protein production host
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, July 2007
|
| DOI | 10.1007/s00253-007-1089-3 |
| Pubmed ID | |
| Authors |
Patricia S. Vary, Rebekka Biedendieck, Tobias Fuerch, Friedhelm Meinhardt, Manfred Rohde, Wolf-Dieter Deckwer, Dieter Jahn |
| Abstract |
Bacillus megaterium has been industrially employed for more than 50 years, as it possesses some very useful and unusual enzymes and a high capacity for the production of exoenzymes. It is also a desirable cloning host for the production of intact proteins, as it does not possess external alkaline proteases and can stably maintain a variety of plasmid vectors. Genetic tools for this species include transducing phages and several hundred mutants covering the processes of biosynthesis, catabolism, division, sporulation, germination, antibiotic resistance, and recombination. The seven plasmids of B. megaterium strain QM B1551 contain several unusual metabolic genes that may be useful in bioremediation. Recently, several recombinant shuttle vectors carrying different strong inducible promoters and various combinations of affinity tags for simple protein purification have been constructed. Leader sequences-mediated export of affinity-tagged proteins into the growth medium was made possible. These plasmids are commercially available. For a broader application of B. megaterium in industry, sporulation and protease-deficient as well as UV-sensitive mutants were constructed. The genome sequence of two different strains, plasmidless DSM319 and QM B1551 carrying seven natural plasmids, is now available. These sequences allow for a systems biotechnology optimization of the production host B. megaterium. Altogether, a "toolbox" of hundreds of genetically characterized strains, genetic methods, vectors, hosts, and genomic sequences make B. megaterium an ideal organism for industrial, environmental, and experimental applications. |
Mendeley readers
The data shown below were compiled from readership statistics for 408 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | <1% |
| Portugal | 1 | <1% |
| Chile | 1 | <1% |
| France | 1 | <1% |
| India | 1 | <1% |
| Germany | 1 | <1% |
| New Zealand | 1 | <1% |
| United Kingdom | 1 | <1% |
| Belgium | 1 | <1% |
| Other | 1 | <1% |
| Unknown | 395 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 73 | 18% |
| Student > Ph. D. Student | 57 | 14% |
| Student > Master | 53 | 13% |
| Researcher | 49 | 12% |
| Student > Doctoral Student | 26 | 6% |
| Other | 47 | 12% |
| Unknown | 103 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 135 | 33% |
| Biochemistry, Genetics and Molecular Biology | 73 | 18% |
| Engineering | 27 | 7% |
| Chemistry | 13 | 3% |
| Immunology and Microbiology | 11 | 3% |
| Other | 34 | 8% |
| Unknown | 115 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2024.
All research outputs
#2,279,831
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#204
of 8,034 outputs
Outputs of similar age
#4,787
of 69,075 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#4
of 65 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 69,075 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 65 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.