| Title |
Macrobrachium rosenbergii nodavirus infection in a giant freshwater prawn hatchery in Indonesia
|
|---|---|
| Published in |
SpringerPlus, October 2016
|
| DOI | 10.1186/s40064-016-3127-z |
| Pubmed ID | |
| Authors |
Murwantoko Murwantoko, Murwantoko Murwantoko, Arif Bimantara, Roosmanto Roosmanto, Masashi Kawaichi |
| Abstract |
A pathogen of giant freshwater prawn, Macrobrachium rosenbergii, was recently recorded in a hatchery in Yogyakarta. The clinical symptom in post-larvae (PL) was a whitish appearance of the muscles in the tail. Histological examination revealed myonecrosis with massive infiltration of myonuclei and hemocytes. RT-PCR products of 850 bp were obtained when using RNA from diseased PL as a template. The clinical signs and RT-PCR amplicon were reproduced in M. rosenbergii inoculated with bacteria-free inocula. Electron microscopy demonstrated that the M. rosenbergii nodavirus (MrNV) was icosahedral in shape and 28.12 ± 2.31 nm in diameter. RT-PCR products of the RNA-dependent RNA polymerase gene (RNA-1) and capsid protein gene (RNA-2) of MrNV were obtained using designed primer pairs, cloned into pBluescript-KS, and sequenced. The 1312 nucleotide (nt) sequence of MrNV RNA-1 revealed 98.0 % identity with isolates from China and India. Additionally, the 1112 nt sequence of MrNV RNA-2 displayed 98.0 % identity with isolates from China and Taiwan. Disease control efforts involving disinfection of PL, broodstocks, water media, tanks, equipment and ponds successfully eradicated white tail disease from the hatchery. This study is the first report on white tail disease and the isolation and characterization of MrNV in Indonesia. |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 43 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 8 | 19% |
| Student > Ph. D. Student | 8 | 19% |
| Researcher | 6 | 14% |
| Student > Bachelor | 4 | 9% |
| Student > Doctoral Student | 2 | 5% |
| Other | 3 | 7% |
| Unknown | 12 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 37% |
| Biochemistry, Genetics and Molecular Biology | 6 | 14% |
| Environmental Science | 2 | 5% |
| Medicine and Dentistry | 2 | 5% |
| Business, Management and Accounting | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 14 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2016.
All research outputs
#20,344,065
of 22,890,496 outputs
Outputs from SpringerPlus
#1,460
of 1,850 outputs
Outputs of similar age
#276,850
of 319,894 outputs
Outputs of similar age from SpringerPlus
#128
of 150 outputs
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