| Title |
Multidrug resistance operon emrAB contributes for chromate and ampicillin co-resistance in a Staphylococcus strain isolated from refinery polluted river bank
|
|---|---|
| Published in |
SpringerPlus, September 2016
|
| DOI | 10.1186/s40064-016-3253-7 |
| Pubmed ID | |
| Authors |
He Zhang, Yantian Ma, Pu Liu, Xiangkai Li |
| Abstract |
EmrAB operon is known for multidrug resistance in bacteria and yet has not been reported related to heavy metal resistance or antibiotics/heavy metal co-resistance. Strain Staphylococcus aureus LZ-01 which was isolated from industrial wastewater discharging site can co-resist to 6 mM Cr(VI) and 0.75 mg/ml ampicillin. Transcriptome data showed that an emrAB operon was upregulated (1.29-folds for emrA, 2.14-folds for emrB) under 0.4 mM Cr(VI) treatment. Quantitative PCR results revealed that this operon was upregulated (1.60-folds for emrA, 2.34-folds for emrB) after 0.20 mg/ml ampicillin treatment. Mutant strain with emrA gene knockout resulted in a 0.83-folds decrease in chromate resistance, and a 0.80-folds decrease in ampicillin resistance; while emrB knockout strain resulted in a 0.33-folds decrease in chromate resistance, and a 0.60-folds decrease in ampicillin resistance. The complemented strains of both deletion mutants basically restored their resistant performance. The presence of 0.50 mM Cr(VI) induced an elevation in ampicillin resistance from 0.50 to 2.50 mg/ml in the strain LZ-01, similarly, its Cr(VI) resistance was also found to be elevated from 6 to 10 mM by 0.15 mg/ml ampicillin induction. The induction effect could be eliminated by deletion of emrA or emrB. Our results demonstrated that the chromosomal emrAB operon in Staphylococcus aureus LZ-01 was a new type of multidrug resistance system, which conferred both ampicillin and chromate resistance to host cells inhabiting polluted environments. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 18% |
| Student > Ph. D. Student | 5 | 15% |
| Student > Bachelor | 4 | 12% |
| Researcher | 2 | 6% |
| Lecturer | 2 | 6% |
| Other | 4 | 12% |
| Unknown | 11 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 18% |
| Agricultural and Biological Sciences | 6 | 18% |
| Environmental Science | 4 | 12% |
| Immunology and Microbiology | 3 | 9% |
| Nursing and Health Professions | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 12 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 October 2016.
All research outputs
#17,817,005
of 22,889,074 outputs
Outputs from SpringerPlus
#1,203
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Outputs of similar age
#230,123
of 321,009 outputs
Outputs of similar age from SpringerPlus
#118
of 172 outputs
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