| Title |
Identification of lipopeptides in Bacillus megaterium by two-step ultrafiltration and LC–ESI–MS/MS
|
|---|---|
| Published in |
AMB Express, September 2016
|
| DOI | 10.1186/s13568-016-0252-6 |
| Pubmed ID | |
| Authors |
Yunxiao Ma, Qing Kong, Chong Qin, Yulin Chen, Yujie Chen, Ruihuan Lv, Guanghui Zhou |
| Abstract |
A two-step ultrafiltration and liquid chromatography-electronic spray ionization-tandem mass spectrometry (LC-ESI-MS/MS) were applied to investigate the nature of lipopeptides in the methanol extract harvested from marine Bacillus megaterium. The structure of lipopeptide homologs were characterized by using collision-induced dissociation mass spectrometry analysis. Collectively, two kinds of linear fengycin A and B with a double bond were characterized for the first time besides the popular cyclic fengycin A and B with the difference in the amino acid at position 6 of the peptide moiety. It is worth noting that two kinds of linear isoforms of surfactins and two kinds of cyclic isoforms of esperin with a smaller peptide ring formed by the fifth L-Asp and β-hydroxy fatty acid were also separated and identified in addition to the all seven kinds of cyclic isoforms of surfactins. Only one analogue (bacillomycin D) of iturin family was identified in this research. To our best knowledge, this is the first report that more than 40 variants of lipopeptides from one strain of Bacillus were identified using our potent purification and identification methods. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 91 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 22% |
| Student > Bachelor | 12 | 13% |
| Student > Master | 10 | 11% |
| Student > Doctoral Student | 8 | 9% |
| Researcher | 8 | 9% |
| Other | 12 | 13% |
| Unknown | 21 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 26% |
| Biochemistry, Genetics and Molecular Biology | 21 | 23% |
| Chemistry | 4 | 4% |
| Immunology and Microbiology | 4 | 4% |
| Chemical Engineering | 3 | 3% |
| Other | 10 | 11% |
| Unknown | 25 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 September 2016.
All research outputs
#14,861,191
of 22,888,307 outputs
Outputs from AMB Express
#347
of 1,236 outputs
Outputs of similar age
#192,979
of 320,716 outputs
Outputs of similar age from AMB Express
#12
of 49 outputs
Altmetric has tracked 22,888,307 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,236 research outputs from this source. They receive a mean Attention Score of 2.8. This one has gotten more attention than average, scoring higher than 64% of its peers.
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We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.