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Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide

Overview of attention for article published in AMB Express, January 2013
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  • Good Attention Score compared to outputs of the same age (69th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (60th percentile)

Mentioned by

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Citations

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129 Dimensions

Readers on

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147 Mendeley
Title
Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide
Published in
AMB Express, January 2013
DOI 10.1186/2191-0855-3-1
Pubmed ID
Authors

Gerard Àlvarez, Marta González, Sergio Isabal, Vanessa Blanc, Rubén León

Abstract

Real-time PCR (qPCR) is a widely used technique in analysing environmental and clinical microbiological samples. However, its main limitation was its inability to discriminate between live and dead cells.Recently, propidium monoazide (PMA) together with qPCR has been used to overcome this problem, with good results for different bacterial species in different types of samples.Our objective was to implement this technique for analysing mortality in multi-species oral biofilms formed in vitro with five oral bacteria: Streptococcus oralis, Streptococcus gordonii, Veillonella parvula, Fusobacterium nucleatum and Prevotella intermedia. We also tested its effectiveness on biofilms treated with an antiseptic solution containing 0.07% w/w cetylpyridinium chloride (CPC).Standardisation of the qPCR-PMA method was performed on pure, heat-killed planktonic cultures of each species, detecting mortality higher than 4 log in S. oralis, S. gordonii and F. nucleatum and higher than 2 for V. parvula and P. intermedia. We obtained similar results for all species when using CPC.When we analysed biofilms with qPCR-PMA, we found that the mortality in the non-CPC treated multi-species biofilms was lower than 1 log for all species. After treatment with CPC, the viability reduction was higher than 4 log in S. oralis and S. gordonii, higher than 3 log in F. nucleatum and P. intermedia and approximately 2 in V. parvula.In short, we standardised the conditions for using qPCR-PMA in 5 oral bacterial species and proved its usefulness for quantification of live and dead cells in multi-species oral biofilms formed in vitro, after use of an antiseptic.

Mendeley readers

The data shown below were compiled from readership statistics for 147 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 147 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 4%
Student > Ph. D. Student 4 3%
Researcher 3 2%
Student > Doctoral Student 3 2%
Student > Bachelor 3 2%
Other 9 6%
Unknown 119 81%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 6%
Pharmacology, Toxicology and Pharmaceutical Science 4 3%
Immunology and Microbiology 4 3%
Agricultural and Biological Sciences 4 3%
Chemistry 3 2%
Other 3 2%
Unknown 120 82%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 July 2021.
All research outputs
#6,245,290
of 19,911,470 outputs
Outputs from AMB Express
#137
of 1,137 outputs
Outputs of similar age
#82,455
of 280,386 outputs
Outputs of similar age from AMB Express
#9
of 25 outputs
Altmetric has tracked 19,911,470 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,137 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,386 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.