The data shown below were compiled from readership statistics for 193 Mendeley readers of this research output. Click here to see the associated Mendeley record.
| Title |
Characterization of two antimicrobial peptides produced by a halotolerant Bacillus subtilis strain SK.DU.4 isolated from a rhizosphere soil sample
|
|---|---|
| Published in |
AMB Express, January 2013
|
| DOI | 10.1186/2191-0855-3-2 |
| Pubmed ID | |
| Authors |
Piyush Baindara, Santi M Mandal, Niharika Chawla, Pradip Kumar Singh, Anil Kumar Pinnaka, Suresh Korpole |
| Abstract |
A bacterial strain producing two antimicrobial peptides was isolated from a rhizosphere soil sample and identified as Bacillus subtilis based on both phenotypic and 16S rRNA gene sequence phylogenetic analysis. It grew optimally up to 14% NaCl and produced antimicrobial peptide within 24 h of growth. The peptides were purified using a combination of chemical extraction and chromatographic techniques. The MALDI-TOF analysis of HPLC purified fractions revealed that the strain SK.DU.4 secreted a bacteriocin-like peptide with molecular mass of 5323.9 Da and a surface-active lipopeptide (m/z 1056 Da). The peptide mass fingerprinting of low-molecular-weight bacteriocin exhibited significant similarity with stretches of secreted lipoprotein of Methylomicrobium album BG8 and displayed 70% sequence coverage. MALDI MS/MS analysis elucidated the lipopeptide as a cyclic lipopeptide with a β-hydroxy fatty acid linked to Ser of a peptide with seven α-amino acids (Asp-Tyr-Asn-Gln-Pro-Asn-Ser) and assigned it to iturin-like group of antimicrobial biosurfactants. However, it differed in amino acid composition with other members of the iturin family. Both peptides were active against Gram-positive bacteria, suggesting that they had an additive effect. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Rwanda | 1 | <1% |
| Unknown | 192 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 42 | 22% |
| Student > Master | 22 | 11% |
| Student > Bachelor | 20 | 10% |
| Researcher | 19 | 10% |
| Student > Doctoral Student | 15 | 8% |
| Other | 33 | 17% |
| Unknown | 42 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 75 | 39% |
| Biochemistry, Genetics and Molecular Biology | 29 | 15% |
| Immunology and Microbiology | 20 | 10% |
| Chemistry | 5 | 3% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
| Other | 14 | 7% |
| Unknown | 47 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 January 2013.
All research outputs
#20,178,031
of 22,691,736 outputs
Outputs from AMB Express
#964
of 1,228 outputs
Outputs of similar age
#249,026
of 281,029 outputs
Outputs of similar age from AMB Express
#9
of 20 outputs
Altmetric has tracked 22,691,736 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,228 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,029 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.