Title |
Cow dung: a potential biomass substrate for the production of detergent-stable dehairing protease by alkaliphilic Bacillus subtilis strain VV
|
---|---|
Published in |
SpringerPlus, December 2012
|
DOI | 10.1186/2193-1801-1-76 |
Pubmed ID | |
Authors |
Ponnuswamy Vijayaraghavan, Aija Vijayan, Arumugaperumal Arun, John Kennady Jenisha, Samuel Gnana Prakash Vincent |
Abstract |
Cow dung, a cheap and easily available source of energy, was used as the substrate for the production of alkaline protease by solid-state fermentation using the Bacillus subtilis strain VV. In order to achieve the maximum yield of this enzyme, the following optimum process parameters are needed: fermentation period (72 h), pH (10.0), moisture content (140%), inoculum (25%), temperature (30-40°C), carbon source (2% (w/w) maltose) and nitrogen source (1% (w/w) urea). The protease was stable over a broad temperature range (30-50°C) and pH (8.0-10.0), with maximum activity at 50°C and pH 10.0. Among the divalent ions tested, Ca(2+) (0.01 M) increased enzyme activity. The purified protease, after being subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis, was found to have a molecular mass of 38.5 kDa. The enzyme was solvent-and surfactant-stable and showed activity even after 24 h incubation along with various commercially available detergents. This enzyme possessed dehairing properties for animal hide after 16 h of incubation at room temperature. From these results it is evident that cow dung is a potential substrate for the production of a detergent-stable, dehairing protease by B. subtilis. This enzyme has a lot of potential applications in the detergent and leather-processing industries. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 1 | 3% |
Unknown | 33 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 29% |
Student > Master | 4 | 12% |
Researcher | 4 | 12% |
Student > Bachelor | 3 | 9% |
Professor | 2 | 6% |
Other | 2 | 6% |
Unknown | 9 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 9 | 26% |
Biochemistry, Genetics and Molecular Biology | 5 | 15% |
Engineering | 3 | 9% |
Environmental Science | 2 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 4 | 12% |
Unknown | 10 | 29% |