Title |
Promotion of the lipase-catalyzed hydrolysis of conjugated linoleic acid l-menthyl ester by addition of an organic solvent
|
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Published in |
SpringerPlus, December 2012
|
DOI | 10.1186/2193-1801-1-67 |
Pubmed ID | |
Authors |
Takashi Kobayashi, Toshihiro Nagao, Yomi Watanabe, Yuji Shimada |
Abstract |
Conjugated linoleic acid l-menthyl ester was hydrolyzed in water by the lipase from Candida rugosa with the addition of an organic solvent. The degree of hydrolysis (yield) greatly improved when a tertiary alcohol, such as t-butyl alcohol, was added. However, the addition of a less polar solvent, such as hexane, decreased the degree of hydrolysis, and some water-miscible solvents, such as acetone, caused inactivation of the lipase. With the addition of t-butyl alcohol, the reaction mixture formed a one- or two-phase system, and the mixing ratio of substrates and t-butyl alcohol determined the number of phases. Although the degree of hydrolysis at 10 d was higher in the one-phase system, the initial reaction rate was generally lower. Meanwhile, the reaction was much faster in the two-phase system while maintaining a moderate degree of hydrolysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 43% |
Student > Master | 2 | 14% |
Other | 1 | 7% |
Unspecified | 1 | 7% |
Unknown | 4 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 29% |
Chemical Engineering | 2 | 14% |
Unspecified | 1 | 7% |
Materials Science | 1 | 7% |
Unknown | 6 | 43% |