| Title |
Inhibition of cancer cell proliferation by 5-fluoro-2'-deoxycytidine, a DNA methylation inhibitor, through activation of DNA damage response pathway
|
|---|---|
| Published in |
SpringerPlus, December 2012
|
| DOI | 10.1186/2193-1801-1-65 |
| Pubmed ID | |
| Authors |
Quanyi Zhao, Jiadong Fan, Wei Hong, Lianyun Li, Min Wu |
| Abstract |
Multiple epigenetic changes, including alterations in DNA methylation occur during tumorigenesis. Various inhibitors of DNA methylation have been developed to prevent proliferation of cancer cells. 5-fluoro-2(')-deoxycytidine (FCdR) is one such DNA methylation inhibitor, which is currently in phase II clinical trial. To investigate the molecular mechanism/s by which FCdR might mediate repression of tumor cell proliferation, we analyzed the toxicity of FCdR in various cell lines established from different sarcomas. We found HCT116, a colon cancer cell line, is much more sensitive to FCdR compared to others. FCdR treatment inhibited HCT116 cells at G2/M check point and up-regulated expression of multiple cancer-related genes, which could be due to its inhibitory activity towards DNA methylation. Furthermore, we found that FCdR activates DNA damage response pathway. Using an inhibitor for ATM and ATR kinases activity, which are required for amplifying the DNA damage repair signal, we show that FCdR induced inhibition of HCT116 cells at G2/M is mediated through activation of DNA damage response pathway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2193-1801-1-65) contains supplementary material, which is available to authorized users. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Hong Kong | 1 | 4% |
| Unknown | 27 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 7 | 25% |
| Student > Master | 6 | 21% |
| Researcher | 4 | 14% |
| Student > Ph. D. Student | 2 | 7% |
| Professor > Associate Professor | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 7 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 25% |
| Biochemistry, Genetics and Molecular Biology | 5 | 18% |
| Immunology and Microbiology | 2 | 7% |
| Engineering | 2 | 7% |
| Chemistry | 2 | 7% |
| Other | 3 | 11% |
| Unknown | 7 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 December 2012.
All research outputs
#20,176,348
of 22,689,790 outputs
Outputs from SpringerPlus
#1,461
of 1,851 outputs
Outputs of similar age
#247,291
of 278,829 outputs
Outputs of similar age from SpringerPlus
#24
of 50 outputs
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