| Title |
Terpenoid bioactive compound from Streptomyces rochei (M32): taxonomy, fermentation and biological activities
|
|---|---|
| Published in |
World Journal of Microbiology and Biotechnology, August 2016
|
| DOI | 10.1007/s11274-016-2121-5 |
| Pubmed ID | |
| Authors |
Raasaiyah Pazhanimurugan, Manikkam Radhakrishnan, Thangavel Shanmugasundaram, Venugopal Gopikrishnan, Ramasamy Balagurunathan |
| Abstract |
The present study emphasized the production of biologically active terpenoid compound from Streptomyces rochei M32, which was isolated from Western Ghats ecosystem, South India. The presence of resistant genes like mecA, vanA of Staphylococcus aureus and bla SHV, bla TEM of Pseudomonas aeruginosa was confirmed by molecular studies. The isolated compound from Streptomyces rochei M32 inhibited wide range of standard and clinical drug resistant pathogens and enteric pathogens. The rice bran supplemented basal medium influenced the active compound production on 8th day of fermentation and yielded 1875 mg of crude extract from 10 g of rice bran substrate. Purification and characterization of crude ethyl acetate extract was achieved by preparative thin layer chromatography. The active fraction was identified as terpenoid class compound by chemical screening. Based on the results of spectral studies (NMR, LC-MS, FTIR, etc.), the active compound was tentatively identified as 1, 19-bis (3-hydroxyazetidin-1-yl) nonadeca-5, 14-diene-1, 8, 12, 19-tetraone with molecular weight 462.41 g/mol. Minimum inhibitory concentration value ranges between 7.6 and 31.2 µg/mL against test organisms was observed. The cytotoxicity results on cervical cancer (HeLa) cell line showed IC50 value of 2.034 µg/mL. The corresponding compound is not previously reported from any microbial resources. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 20% |
| Student > Bachelor | 2 | 8% |
| Student > Master | 2 | 8% |
| Professor | 2 | 8% |
| Researcher | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 10 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 16% |
| Biochemistry, Genetics and Molecular Biology | 3 | 12% |
| Medicine and Dentistry | 2 | 8% |
| Immunology and Microbiology | 2 | 8% |
| Earth and Planetary Sciences | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 11 | 44% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 August 2016.
All research outputs
#15,687,152
of 23,911,072 outputs
Outputs from World Journal of Microbiology and Biotechnology
#928
of 1,757 outputs
Outputs of similar age
#212,521
of 344,854 outputs
Outputs of similar age from World Journal of Microbiology and Biotechnology
#10
of 24 outputs
Altmetric has tracked 23,911,072 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,757 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 344,854 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.