| Title |
Extracellular expression of glutamate decarboxylase B in Escherichia coli to improve gamma-aminobutyric acid production
|
|---|---|
| Published in |
AMB Express, August 2016
|
| DOI | 10.1186/s13568-016-0231-y |
| Pubmed ID | |
| Authors |
Anqi Zhao, Xiaoqing Hu, Ye Li, Cheng Chen, Xiaoyuan Wang |
| Abstract |
Escherichia coli overexpressing glutamate decarboxylase GadB can produce gamma-aminobutyric acid with addition of monosodium glutamate. The yield and productivity of gamma-aminobutyric acid might be significantly improved if the overexpressed GadB in E. coli cells can be excreted outside, where it can directly transforms monosodium glutamate to gamma-aminobutyric acid. In this study, GadB was fused to signal peptides TorA or PelB, respectively, and overexpressed in E. coli BL21(DE3). It was found that TorA could facilitate GadB secretion much better than PelB. Conditions for GadB secretion and gamma-aminobutyric acid production were optimized in E. coli BL21(DE3)/pET20b-torA-gadB, leading the secretion of more than half of the overexpressed GadB. Fed-batch fermentation for GadB expression and gamma-aminobutyric acid production of BL21(DE3)/pET20b-torA-gadB was sequentially performed in one fermenter; 264.4 and 313.1 g/L gamma-aminobutyric acid were obtained with addition of monosodium glutamate after 36 and 72 h, respectively. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 29 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 17% |
| Student > Bachelor | 3 | 10% |
| Student > Postgraduate | 3 | 10% |
| Student > Master | 3 | 10% |
| Researcher | 2 | 7% |
| Other | 3 | 10% |
| Unknown | 10 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 24% |
| Agricultural and Biological Sciences | 4 | 14% |
| Immunology and Microbiology | 2 | 7% |
| Chemical Engineering | 1 | 3% |
| Medicine and Dentistry | 1 | 3% |
| Other | 2 | 7% |
| Unknown | 12 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2016.
All research outputs
#15,381,416
of 22,883,326 outputs
Outputs from AMB Express
#446
of 1,236 outputs
Outputs of similar age
#219,536
of 343,744 outputs
Outputs of similar age from AMB Express
#27
of 47 outputs
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