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Extracellular expression of glutamate decarboxylase B in Escherichia coli to improve gamma-aminobutyric acid production

Overview of attention for article published in AMB Express, August 2016
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1 tweeter

Citations

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Readers on

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23 Mendeley
Title
Extracellular expression of glutamate decarboxylase B in Escherichia coli to improve gamma-aminobutyric acid production
Published in
AMB Express, August 2016
DOI 10.1186/s13568-016-0231-y
Pubmed ID
Authors

Anqi Zhao, Anqi Zhao, Xiaoqing Hu, Ye Li, Cheng Chen, Xiaoyuan Wang

Abstract

Escherichia coli overexpressing glutamate decarboxylase GadB can produce gamma-aminobutyric acid with addition of monosodium glutamate. The yield and productivity of gamma-aminobutyric acid might be significantly improved if the overexpressed GadB in E. coli cells can be excreted outside, where it can directly transforms monosodium glutamate to gamma-aminobutyric acid. In this study, GadB was fused to signal peptides TorA or PelB, respectively, and overexpressed in E. coli BL21(DE3). It was found that TorA could facilitate GadB secretion much better than PelB. Conditions for GadB secretion and gamma-aminobutyric acid production were optimized in E. coli BL21(DE3)/pET20b-torA-gadB, leading the secretion of more than half of the overexpressed GadB. Fed-batch fermentation for GadB expression and gamma-aminobutyric acid production of BL21(DE3)/pET20b-torA-gadB was sequentially performed in one fermenter; 264.4 and 313.1 g/L gamma-aminobutyric acid were obtained with addition of monosodium glutamate after 36 and 72 h, respectively.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 22%
Student > Postgraduate 3 13%
Student > Bachelor 3 13%
Professor > Associate Professor 2 9%
Researcher 2 9%
Other 2 9%
Unknown 6 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 26%
Agricultural and Biological Sciences 3 13%
Immunology and Microbiology 2 9%
Chemical Engineering 1 4%
Medicine and Dentistry 1 4%
Other 2 9%
Unknown 8 35%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2016.
All research outputs
#4,392,407
of 8,261,086 outputs
Outputs from AMB Express
#217
of 493 outputs
Outputs of similar age
#139,847
of 253,846 outputs
Outputs of similar age from AMB Express
#24
of 48 outputs
Altmetric has tracked 8,261,086 research outputs across all sources so far. This one is in the 27th percentile – i.e., 27% of other outputs scored the same or lower than it.
So far Altmetric has tracked 493 research outputs from this source. They receive a mean Attention Score of 2.0. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 253,846 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 48 others from the same source and published within six weeks on either side of this one. This one is in the 2nd percentile – i.e., 2% of its contemporaries scored the same or lower than it.