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miR-125b-1 is repressed by histone modifications in breast cancer cell lines

Overview of attention for article published in SpringerPlus, July 2016
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4 tweeters

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32 Mendeley
Title
miR-125b-1 is repressed by histone modifications in breast cancer cell lines
Published in
SpringerPlus, July 2016
DOI 10.1186/s40064-016-2475-z
Pubmed ID
Authors

Cisneros-Soberanis, Fernanda, Andonegui, Marco A, Herrera, Luis A, Fernanda Cisneros-Soberanis, Marco A. Andonegui, Luis A. Herrera

Abstract

Downregulation of miR-125b-1 is associated with poor prognosis in breast cancer patients. In this work we investigated the effect of histone modifications on the regulation of this gene promoter. We evaluated the enrichment of two histone modifications involved in gene repression, H3K9me3 and H3K27me3, on the miR-125b-1 promoter in two breast cancer cell lines, MCF7 (luminal A subtype) and MDA-MB-231 (triple-negative subtype), compared to the non-transformed breast cell line MCF10A. H3K27me3 and H3K9me3 were enriched in MCF7 and MDA-MB-231 cells, respectively. Next, we used an EZH2 inhibitor to examine the reactivation of miR-125b-1 in MCF7 cells and evaluated the transcriptional levels of pri-miR-125b-1 and mature miR-125b by qRT-PCR. pri-miRNA and mature miRNA transcripts were both increased after treatment of MCF7 cells with the EZH2 inhibitor, whereas no effect on miR-125b-1 expression levels was observed in MDA-MB-231 and MCF10A cells. We subsequently evaluated the effect of miR-125b-1 reactivation on the expression and protein levels of BAK1, a target of miR-125b. We observed 60 and 70 % decreases in the expression and protein levels of BAK1, respectively, compared to cells that were not treated with the EZH2 inhibitor. We over-expressed KDM4B/JMJD2B to reactivate this miRNA, resulting in a three-fold increase in miR-125b expression compared with the same cell line without KDM4B/JMJD2B over-expression. The miR-125b-1 is repressed by different epigenetic mechanisms depending on the breast cancer subtype and that miR-125b-1 reactivation specifically eliminates the effect of repressive histone modifications on the expression of an pro-apoptotic target.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 22%
Student > Master 5 16%
Researcher 5 16%
Student > Bachelor 2 6%
Student > Postgraduate 2 6%
Other 5 16%
Unknown 6 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 44%
Medicine and Dentistry 7 22%
Chemistry 3 9%
Agricultural and Biological Sciences 1 3%
Immunology and Microbiology 1 3%
Other 1 3%
Unknown 5 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 July 2016.
All research outputs
#12,684,641
of 19,247,712 outputs
Outputs from SpringerPlus
#895
of 1,818 outputs
Outputs of similar age
#154,895
of 269,775 outputs
Outputs of similar age from SpringerPlus
#26
of 46 outputs
Altmetric has tracked 19,247,712 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,818 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,775 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.