RT @DevoEvoMed: @biorxivpreprint One of the nice things about RNA-Seq data is getting gene expression (transcript abundance) estimates, whe…
@biorxivpreprint One of the nice things about RNA-Seq data is getting gene expression (transcript abundance) estimates, whether that's in RPKM, FPKM, CPM, or TPM. We and others have shown you really should use TPM... https://t.co/AWop4VuTnG
TPMの計算式を調べなおしてみたら、ずっと勘違いしてたことに気づいた。(最後にcancel out されるけど、式の上ではタグの平均長の項が入ってたんですね) https://t.co/InaLDATYKY
https://t.co/R1V4xE2ndn … The guide for the difference between RPKM, FPKM, and TPM. and https://t.co/J79rI58dNC
RT @carloshotta: O pior chato é o que está certo: RPKM é uma medida inconsistente entre amostras https://t.co/L5ZlgQifrq
O pior chato é o que está certo: RPKM é uma medida inconsistente entre amostras https://t.co/L5ZlgQifrq
Later Günter Wagner @yale_eeb, who had his own ideas on this topic (https://t.co/tz2nKiXFRG), challenged me on this point. This made me want to put normalization methods to the test to find out for sure.
Maybe you don't need RPKM. https://t.co/TsA6DMcKyn https://t.co/4UARAlG7Xc
@blekhman use distribution of TPMs in sample, usually ends up TPM<2 is not expressed https://t.co/bHoX4xEYYg
@coletrapnell @CamLBerthelot @hjpimentel Even if normalized RPKM inconsistent across samples: http://t.co/C5drqkyYgp https://t.co/1pj6EWBfIF
@jxtx @jmchilton @IPythonDev for proofs see http://t.co/mKmgdP2bDo, http://t.co/P6680WMWmY, etc.
Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples http://t.co/1zb9Y4xm
No rpkm. Raw count n deseq/edgeR “@genetics_blog: RNA-seq data: RPKM measure is inconsistent among samples http://t.co/iwkzRJVB” #genomics
Measurement of #mRNA abundance using #RNA-seq da... [Theory Biosci. 2012] - PubMed - NCBI:... http://t.co/4slTxe8D #rnomics_bioinfo @MyEN
Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples http://t.co/yaz8t4wB
Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples http://t.co/yaz8t4wB
Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples http://t.co/yaz8t4wB
RPKM measure is inconsistent among samples http://t.co/n5cvK9Hs
RPKM measure is inconsistent among samples http://t.co/5nCBIuNQ
Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples http://t.co/yaz8t4wB
Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples http://t.co/yaz8t4wB