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P2X7 receptor activation mediates organic cation uptake into human myeloid leukaemic KG-1 cells

Overview of attention for article published in Purinergic Signalling, June 2012
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Title
P2X7 receptor activation mediates organic cation uptake into human myeloid leukaemic KG-1 cells
Published in
Purinergic Signalling, June 2012
DOI 10.1007/s11302-012-9320-9
Pubmed ID
Authors

Safina Gadeock, Aleta Pupovac, Vanessa Sluyter, Mari Spildrejorde, Ronald Sluyter

Abstract

The P2X7 purinergic receptor is an ATP-gated cation channel with an emerging role in neoplasia. In this study we demonstrate that the human KG-1 cell line, a model of acute myelogenous leukaemia, expresses functional P2X7. RT-PCR and immunochemical techniques demonstrated the presence of P2X7 mRNA and protein respectively in KG-l cells, as well as in positive control multiple myeloma RPMI 8226 cells. Flow cytometric measurements demonstrated that ATP induced ethidium(+) uptake into KG-l cells suspended in sucrose medium (EC(50) of ≈ 3 μM), but not into cells in NaCl medium. In contrast, ATP induced ethidium(+) uptake into RPMI 8226 cells suspended in either sucrose or NaCl medium (EC(50) of ≈ 3 or ≈ 99 μM, respectively), as well as into RPMI 8226 cells in KCl medium (EC(50) of ≈ 18 μM). BzATP and to a lesser extent ATPγS and αβ-methylene ATP, but not ADP or UTP, also induced ethidium(+) uptake into KG-1 cells. ATP-induced ethidium(+) uptake was completely impaired by the P2X7 antagonists, AZ10606120 and A-438079. ATP-induced ethidium(+) uptake was also impaired by probenecid but not by carbenoxolone, both pannexin-1 antagonists. ATP induced YO-PRO-1(2+) and propidium(2+) uptake into KG-1 cells. Finally, sequencing of full-length P2X7 cDNA identified several single nucleotide polymorphisms (SNPs) in KG-1 cells including H155Y, A348T, T357S and Q460R. RPMI 8226 cells contained A348T, A433V and H521Q SNPs. In conclusion, the KG-1 cell line expresses functional P2X7. This cell line may help elucidate the signalling pathways involved in P2X7-induced survival and invasiveness of myeloid leukaemic cells.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 6%
Unknown 16 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 35%
Researcher 3 18%
Student > Bachelor 2 12%
Student > Postgraduate 2 12%
Student > Doctoral Student 2 12%
Other 2 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 29%
Agricultural and Biological Sciences 4 24%
Medicine and Dentistry 3 18%
Unspecified 2 12%
Immunology and Microbiology 1 6%
Other 2 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 June 2012.
All research outputs
#2,897,977
of 3,632,901 outputs
Outputs from Purinergic Signalling
#35
of 63 outputs
Outputs of similar age
#55,175
of 74,870 outputs
Outputs of similar age from Purinergic Signalling
#2
of 4 outputs
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So far Altmetric has tracked 63 research outputs from this source. They receive a mean Attention Score of 1.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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