Title |
Lectin binding studies on a glycopolymer brush flow-through biosensor by localized surface plasmon resonance
|
---|---|
Published in |
Analytical & Bioanalytical Chemistry, June 2016
|
DOI | 10.1007/s00216-016-9667-9 |
Pubmed ID | |
Authors |
Ruben R. Rosencrantz, Vu Hoa Nguyen, Hyunji Park, Christine Schulte, Alexander Böker, Uwe Schnakenberg, Lothar Elling |
Abstract |
A localized surface plasmon resonance biosensor in a flow-through configuration was applied for investigating kinetics of lectin binding to surface-grafted glycopolymer brushes. Polycarbonate filter membranes with pore sizes of 400 nm were coated with a 114-nm thick gold layer and used as substrate for surface-initiated atom-transfer radical polymerization of a glycomonomer. These grafted from glycopolymer brushes were further modified with two subsequent enzymatic reactions on the surface to yield an immobilized trisaccharide presenting brush. Specific binding of lectins including Clostridium difficile toxin A receptor domain to the glycopolymer brush surface could be investigated in a microfluidic setup with flow-through of the analytes and transmission surface plasmon resonance spectroscopy. Graphical abstract Glycopolymer brushes serve as high affinity ligands for lectin and toxin interactions in a sensitive, disposable flow-through LSPR biosensor. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Ghana | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 10 | 33% |
Student > Master | 2 | 7% |
Professor | 2 | 7% |
Professor > Associate Professor | 2 | 7% |
Researcher | 2 | 7% |
Other | 1 | 3% |
Unknown | 11 | 37% |
Readers by discipline | Count | As % |
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Chemistry | 4 | 13% |
Materials Science | 3 | 10% |
Medicine and Dentistry | 3 | 10% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Engineering | 2 | 7% |
Other | 4 | 13% |
Unknown | 12 | 40% |