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Metabolome analysis of Saccharomyces cerevisiae and optimization of culture medium for S-adenosyl-l-methionine production

Overview of attention for article published in AMB Express, June 2016
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (51st percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

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2 tweeters
facebook
1 Facebook page

Citations

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14 Dimensions

Readers on

mendeley
40 Mendeley
Title
Metabolome analysis of Saccharomyces cerevisiae and optimization of culture medium for S-adenosyl-l-methionine production
Published in
AMB Express, June 2016
DOI 10.1186/s13568-016-0210-3
Pubmed ID
Authors

Kenshi Hayakawa, Fumio Matsuda, Hiroshi Shimizu, Hayakawa, Kenshi, Matsuda, Fumio, Shimizu, Hiroshi

Abstract

S-Adenosyl-L-methionine (SAM) is a fine chemical used as a nutritional supplement and a prescription drug. It is industrially produced using Saccharomyces cerevisiae owing to its high SAM content. To investigate the optimization of culture medium components for higher SAM production, metabolome analysis was conducted to compare the intracellular metabolite concentrations between Kyokai no. 6 (high SAM-producing) and laboratory yeast S288C (control) under different SAM production conditions. Metabolome analysis and the result of principal component analysis showed that the rate-limiting step for SAM production was ATP supply and the levels of degradation products of adenosine nucleotides were higher in Kyokai 6 strain than in the S288C strain under the L-methionine supplemented condition. Analysis of ATP accumulation showed that the levels of intracellular ATP in the Kyokai 6 strain were also higher compared to those in the S288C strain. Furthermore, as expected from metabolome analysis, the SAM content of Kyokai 6 strain cultivated in the medium without yeast extract increased by 2.5-fold compared to that in the additional condition, by increasing intracellular ATP level with inhibited cell growth. These results suggest that high SAM production is attributed to the enhanced ATP supply with L-methionine condition and high efficiency of intracellular ATP consumption.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 40 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 30%
Student > Ph. D. Student 7 18%
Student > Master 5 13%
Other 4 10%
Student > Doctoral Student 3 8%
Other 5 13%
Unknown 4 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 28%
Agricultural and Biological Sciences 9 23%
Chemistry 4 10%
Environmental Science 2 5%
Medicine and Dentistry 2 5%
Other 5 13%
Unknown 7 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 June 2016.
All research outputs
#3,694,870
of 7,936,934 outputs
Outputs from AMB Express
#105
of 462 outputs
Outputs of similar age
#122,158
of 269,273 outputs
Outputs of similar age from AMB Express
#7
of 17 outputs
Altmetric has tracked 7,936,934 research outputs across all sources so far. This one has received more attention than most of these and is in the 51st percentile.
So far Altmetric has tracked 462 research outputs from this source. They receive a mean Attention Score of 2.0. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,273 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.