| Title |
Identification of interleukin genes in Pogona vitticeps using a de novo transcriptome assembly from RNA-seq data
|
|---|---|
| Published in |
Immunogenetics, June 2016
|
| DOI | 10.1007/s00251-016-0922-1 |
| Pubmed ID | |
| Authors |
Alexandra Livernois, Kristine Hardy, Renae Domaschenz, Alexie Papanicolaou, Arthur Georges, Stephen D Sarre, Sudha Rao, Tariq Ezaz, Janine E Deakin |
| Abstract |
Interleukins are a group of cytokines with complex immunomodulatory functions that are important for regulating immunity in vertebrate species. Reptiles and mammals last shared a common ancestor more than 350 million years ago, so it is not surprising that low sequence identity has prevented divergent interleukin genes from being identified in the central bearded dragon lizard, Pogona vitticeps, in its genome assembly. To determine the complete nucleotide sequences of key interleukin genes, we constructed full-length transcripts, using the Trinity platform, from short paired-end read RNA sequences from stimulated spleen cells. De novo transcript reconstruction and analysis allowed us to identify interleukin genes that are missing from the published P. vitticeps assembly. Identification of key cytokines in P. vitticeps will provide insight into the essential molecular mechanisms and evolution of interleukin gene families and allow for characterization of the immune response in a lizard for comparison with mammals. |
X Demographics
The data shown below were collected from the profiles of 12 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 3 | 25% |
| United Kingdom | 1 | 8% |
| France | 1 | 8% |
| Unknown | 7 | 58% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 67% |
| Scientists | 3 | 25% |
| Science communicators (journalists, bloggers, editors) | 1 | 8% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 4% |
| Unknown | 23 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 21% |
| Professor > Associate Professor | 3 | 13% |
| Lecturer > Senior Lecturer | 2 | 8% |
| Student > Ph. D. Student | 2 | 8% |
| Student > Doctoral Student | 2 | 8% |
| Other | 3 | 13% |
| Unknown | 7 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 46% |
| Biochemistry, Genetics and Molecular Biology | 3 | 13% |
| Unspecified | 1 | 4% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Neuroscience | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 7 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 May 2017.
All research outputs
#4,617,000
of 25,066,230 outputs
Outputs from Immunogenetics
#127
of 1,240 outputs
Outputs of similar age
#73,791
of 346,565 outputs
Outputs of similar age from Immunogenetics
#2
of 18 outputs
Altmetric has tracked 25,066,230 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,240 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done well, scoring higher than 89% of its peers.
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We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.