| Title |
A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans
|
|---|---|
| Published in |
Planta, December 2001
|
| DOI | 10.1007/s004250100606 |
| Pubmed ID | |
| Authors |
Filomena A. Pettolino, Nicholas J. Hoogenraad, Charles Ferguson, Antony Bacic, Elizabeth Johnson, Bruce A. Stone |
| Abstract |
Galactomannan was coupled to a protein carrier for the preparation of monoclonal antibodies. The monoclonal antibodies generated bound to galactomannans from different sources as well as to glucomannan and galactoglucomannan. One monoclonal antibody, BGM C6, was characterised and found to be specific for (1-->4)-beta-linked mannopyranosyl residues; it had a binding affinity estimated at 1x10(-6) M for the (1-->4)-beta-linked mannohexaose. BGM C6 was used in immunogold labelling studies to locate galactomannans in the endosperm walls of normal coconuts (Cocos nucifera L.) and those of the mutant makapuno at two different developmental stages. The pattern and intensity of antibody labelling varied for each type of coconut at the mature and immature stages, indicating differences in the galactomannan composition of the endosperm walls. |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 2% |
| Denmark | 1 | 2% |
| Unknown | 53 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 25% |
| Student > Master | 11 | 20% |
| Researcher | 5 | 9% |
| Student > Postgraduate | 5 | 9% |
| Other | 4 | 7% |
| Other | 8 | 15% |
| Unknown | 8 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 29 | 53% |
| Biochemistry, Genetics and Molecular Biology | 6 | 11% |
| Chemistry | 5 | 9% |
| Chemical Engineering | 2 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Other | 4 | 7% |
| Unknown | 8 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2013.
All research outputs
#7,482,726
of 22,873,031 outputs
Outputs from Planta
#599
of 2,720 outputs
Outputs of similar age
#30,017
of 124,424 outputs
Outputs of similar age from Planta
#2
of 12 outputs
Altmetric has tracked 22,873,031 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,720 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.