| Title |
Construction of a novel Pichia pastoris strain for production of xanthophylls
|
|---|---|
| Published in |
AMB Express, April 2012
|
| DOI | 10.1186/2191-0855-2-24 |
| Pubmed ID | |
| Authors |
José Miguel Araya-Garay, José M Ageitos, Juan A Vallejo, Patricia Veiga-Crespo, Angeles Sánchez-Pérez, Tomás G Villa |
| Abstract |
In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-strains harboring astaxanthin-encoding genes from carotenogenic microorganism, and thus capable of producing xanthophyllic compounds. We designed and constructed a plasmid (pGAPZA-WZ) containing both the β-carotene ketolase (crtW) and β-carotene hydroxylase (crtZ) genes from Agrobacterium aurantiacum, under the control of the GAP promoter and containing an AOX-1 terminator. The plasmid was then integrated into the P. pastoris Pp-EBIL strain genomic DNA, producing clone Pp-EBILWZ. The recombinant P. pastoris (Pp-EBILWZ) cells exhibited a strong reddish carotenoid coloration and were confirmed, by HPLC, to produce not only the previous described carotenoids lycopene and β-carotene, but also de novo synthesized astaxanthin. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 102 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Austria | 2 | 2% |
| Japan | 1 | <1% |
| Unknown | 99 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 22 | 22% |
| Student > Ph. D. Student | 18 | 18% |
| Student > Master | 17 | 17% |
| Student > Bachelor | 8 | 8% |
| Other | 6 | 6% |
| Other | 16 | 16% |
| Unknown | 15 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 39 | 38% |
| Biochemistry, Genetics and Molecular Biology | 28 | 27% |
| Chemical Engineering | 3 | 3% |
| Computer Science | 3 | 3% |
| Chemistry | 3 | 3% |
| Other | 6 | 6% |
| Unknown | 20 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 July 2023.
All research outputs
#6,378,944
of 22,664,644 outputs
Outputs from AMB Express
#124
of 1,227 outputs
Outputs of similar age
#44,225
of 163,313 outputs
Outputs of similar age from AMB Express
#1
of 7 outputs
Altmetric has tracked 22,664,644 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 1,227 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 163,313 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them