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Construction of a novel Pichia pastoris strain for production of xanthophylls

Overview of attention for article published in AMB Express, April 2012
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (71st percentile)

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1 tweeter
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2 patents

Citations

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89 Mendeley
Title
Construction of a novel Pichia pastoris strain for production of xanthophylls
Published in
AMB Express, April 2012
DOI 10.1186/2191-0855-2-24
Pubmed ID
Authors

José Miguel Araya-Garay, José M Ageitos, Juan A Vallejo, Patricia Veiga-Crespo, Angeles Sánchez-Pérez, Tomás G Villa

Abstract

In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-strains harboring astaxanthin-encoding genes from carotenogenic microorganism, and thus capable of producing xanthophyllic compounds. We designed and constructed a plasmid (pGAPZA-WZ) containing both the β-carotene ketolase (crtW) and β-carotene hydroxylase (crtZ) genes from Agrobacterium aurantiacum, under the control of the GAP promoter and containing an AOX-1 terminator. The plasmid was then integrated into the P. pastoris Pp-EBIL strain genomic DNA, producing clone Pp-EBILWZ. The recombinant P. pastoris (Pp-EBILWZ) cells exhibited a strong reddish carotenoid coloration and were confirmed, by HPLC, to produce not only the previous described carotenoids lycopene and β-carotene, but also de novo synthesized astaxanthin.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 89 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Austria 2 2%
Japan 1 1%
Unknown 86 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 20 22%
Student > Master 16 18%
Student > Ph. D. Student 12 13%
Other 6 7%
Student > Bachelor 6 7%
Other 16 18%
Unknown 13 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 37 42%
Biochemistry, Genetics and Molecular Biology 25 28%
Chemical Engineering 3 3%
Engineering 2 2%
Computer Science 2 2%
Other 4 4%
Unknown 16 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2021.
All research outputs
#5,596,070
of 20,048,249 outputs
Outputs from AMB Express
#108
of 1,138 outputs
Outputs of similar age
#37,213
of 139,170 outputs
Outputs of similar age from AMB Express
#1
of 2 outputs
Altmetric has tracked 20,048,249 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 1,138 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 139,170 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them