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Generation of Human Embryonic Stem Cell Line Expressing zsGreen in Cholinergic Neurons Using CRISPR/Cas9 System

Overview of attention for article published in Neurochemical Research, April 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

Mentioned by

blogs
1 blog
twitter
1 tweeter

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
13 Mendeley
Title
Generation of Human Embryonic Stem Cell Line Expressing zsGreen in Cholinergic Neurons Using CRISPR/Cas9 System
Published in
Neurochemical Research, April 2016
DOI 10.1007/s11064-016-1918-9
Pubmed ID
Authors

Jing Zhou, Chencheng Wang, Kunshan Zhang, Yingying Wang, Xi Gong, Yanlu Wang, Siguang Li, Yuping Luo

Abstract

Lineage specific human embryonic stem cell (hESC) reporter cell line is a versatile tool for biological studies on real time monitoring of differentiation, physiological and biochemical features of special cell types and pathological mechanism of disease. Here we report the generation of ChAT-zsGreen reporter hESC line that express zsGreen under the control of the choline acetyltransferase (ChAT) promoter using CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats)/Cas9 system. We show that the ChAT-zsGreen hESC reporter cell lines retain the features of undifferentiated hESC. After cholinergic neuronal differentiation, cholinergic neurons were clearly labeled with green fluorescence protein (zsGreen). The ChAT-zsGreen reporter hESC lines are invaluable not only for the monitoring cholinergic neuronal differentiation but also for study physiological and biochemical hallmarks of cholinergic neurons.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 31%
Student > Bachelor 3 23%
Researcher 2 15%
Other 2 15%
Student > Postgraduate 1 8%
Other 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 46%
Agricultural and Biological Sciences 3 23%
Pharmacology, Toxicology and Pharmaceutical Science 1 8%
Immunology and Microbiology 1 8%
Chemistry 1 8%
Other 1 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 August 2016.
All research outputs
#1,812,205
of 12,330,736 outputs
Outputs from Neurochemical Research
#115
of 1,329 outputs
Outputs of similar age
#53,717
of 274,489 outputs
Outputs of similar age from Neurochemical Research
#13
of 74 outputs
Altmetric has tracked 12,330,736 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,329 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 274,489 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 74 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.