Title |
EmmdR, a new member of the MATE family of multidrug transporters, extrudes quinolones from Enterobacter cloacae
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Published in |
Archives of Microbiology, August 2011
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DOI | 10.1007/s00203-011-0738-1 |
Pubmed ID | |
Authors |
Gui-Xin He, Conner Thorpe, Dennis Walsh, Robert Crow, Huizhong Chen, Sanath Kumar, Manuel F. Varela |
Abstract |
We cloned a gene, ECL_03329, from the chromosome of Enterobacter cloacae ATCC13047, using a drug-hypersensitive Escherichia coli KAM32 cell as the host. We show here that this gene, designated as emmdR, is responsible for multidrug resistance in E. cloacae. E. coli KAM32 host cells containing the cloned emmdR gene (KAM32/pEMMDR28) showed decreased susceptibilities to benzalkonium chloride, norfloxacin, ciprofloxacin, levofloxacin, ethidium bromide, acriflavine, rhodamine6G, and trimethoprim. emmdR-deficient E. cloacae cells (EcΔemmdR) showed increased susceptibilities to several of the antimicrobial agents tested. EmmdR has twelve predicted transmembrane segments and some shared identity with members of the multidrug and toxic compound extrusion (MATE) family of transporters. Study of the antimicrobial agent efflux activities revealed that EmmdR is an H+-drug antiporter but not a Na+ driven efflux pump. These results indicate that EmmdR is responsible for multidrug resistance and pumps out quinolones from E. cloacae. |
X Demographics
Geographical breakdown
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United States | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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India | 2 | 4% |
Unknown | 43 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 8 | 18% |
Researcher | 5 | 11% |
Professor > Associate Professor | 4 | 9% |
Student > Doctoral Student | 4 | 9% |
Student > Bachelor | 4 | 9% |
Other | 11 | 24% |
Unknown | 9 | 20% |
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Pharmacology, Toxicology and Pharmaceutical Science | 3 | 7% |
Other | 3 | 7% |
Unknown | 8 | 18% |