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Fluorescence imaging of in vivo miR-124a-induced neurogenesis of neuronal progenitor cells using neuron-specific reporters

Overview of attention for article published in EJNMMI Research, April 2016
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About this Attention Score

  • Among the highest-scoring outputs from this source (#38 of 167)
  • Average Attention Score compared to outputs of the same age
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

Mentioned by

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2 tweeters

Citations

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6 Dimensions

Readers on

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21 Mendeley
Title
Fluorescence imaging of in vivo miR-124a-induced neurogenesis of neuronal progenitor cells using neuron-specific reporters
Published in
EJNMMI Research, April 2016
DOI 10.1186/s13550-016-0190-y
Pubmed ID
Authors

Jaeho Jang, Song Lee, Hyun Jeong Oh, Yoori Choi, Jae Hyouk Choi, Do Won Hwang, Dong Soo Lee

Abstract

Facilitation of the differentiation of the stem cells toward neuronal lineage is crucial for enhancing the differentiation efficacy of grafted stem cells for the possible treatment of neurodegenerative disorders. MicroRNA124a (miR-124a) has been considered as a neuronal lineage regulator, possessing the capability to activate neuronal differentiation. In this study, using a neuronal promoter-based reporter and live-cell fluorescence imaging, we visualized in vitro and in vivo the enhanced neuronal differentiation of neuronal progenitor cells with miR-124a overproduction. The neuron specific alpha1 tubulin promoter-driven RFP reporter (pTa1-RFP) was used to trace the miR-124a-induced neuronal differentiation in live cell condition. MiR-124a or miR-scramble in 10 % glucose buffer was mixed with in vivo-jetPEITM and in vivo fluorescence images were obtained daily using Maestro spectral fluorescent imager. Neurite outgrowth was clearly seen in F11 cells after miR-124a transfection, and immunofluorescence staining showed increase of Tuj1 and NF at 48 hours. When pTa1-RFP-transfected F11 cells were implanted simultaneously with miR-124a into the nude mice, gradually increasing reporter signals and morphological changes indicated neuronal differentiation for 48 hours in live cells in vitro. The miR-124a-treated F11 cells showed higher reporter signals on in vivo fluorescence imaging than miR-scramble-treated cells, which were verified by ex vivo confirmation of Tuj1 and NF expression. These results indicated that neuronal reporter-based neurogenesis imaging can be used for monitoring miR-124a acting as neuronal activator when miRNA was injected in in vivo PEI-coated form for miRNA-mediated regenerative therapy.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 33%
Student > Master 3 14%
Student > Doctoral Student 2 10%
Other 2 10%
Professor 1 5%
Other 4 19%
Unknown 2 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 29%
Neuroscience 4 19%
Biochemistry, Genetics and Molecular Biology 2 10%
Medicine and Dentistry 2 10%
Philosophy 1 5%
Other 4 19%
Unknown 2 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 April 2016.
All research outputs
#3,718,041
of 7,615,205 outputs
Outputs from EJNMMI Research
#38
of 167 outputs
Outputs of similar age
#133,390
of 267,040 outputs
Outputs of similar age from EJNMMI Research
#2
of 7 outputs
Altmetric has tracked 7,615,205 research outputs across all sources so far. This one is in the 48th percentile – i.e., 48% of other outputs scored the same or lower than it.
So far Altmetric has tracked 167 research outputs from this source. They receive a mean Attention Score of 1.4. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,040 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.