| Title |
High level expression of bikunin in Pichia pastoris by fusion of human serum albumin
|
|---|---|
| Published in |
AMB Express, February 2012
|
| DOI | 10.1186/2191-0855-2-14 |
| Pubmed ID | |
| Authors |
Xing-Hua Gou, Yu-Ying Liu, Qi-Lei Chen, Jian-Jun Tang, Da-Yu Liu, Liang Zou, Xiao-Yong Wu, Wei Wang |
| Abstract |
Bikunin is a proteoglycan exhibiting broad-spectrum inhibitory activity against serine proteases and could potentially suppress tumor cell invasion and metastasis. Here, we have successfully expressed recombinant human bikunin (rh-bikunin) in Pichia pastoris and also established the purification procedure. Different fusion genes of h-UTI and domain I, domain I and domain II, domain I, domain II and domain III of human serum albumin (HSA) were inserted into expression vector pPICZαA. After expressed in shake flask, rh-bikunin was produced in an 30-L fermenter and purified by affinity chromatography and cation exchange chromatography. The final expression levels were 200 mg/L and we got totally 1.08 g (3650 IU/mg) of active purified rh-bikunin (purity is 98%) from 20 L of fermentation broth. The rh-bikunin consists of unique form with molecular masses of 25 kDa, and has the same N-terminals sequence as human native bikunin. This study provided a new method for high level expression of active rh-bikunin by using HSA as fusion parter. |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 3% |
| Italy | 1 | 3% |
| Unknown | 29 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 23% |
| Researcher | 6 | 19% |
| Student > Master | 5 | 16% |
| Student > Bachelor | 2 | 6% |
| Other | 2 | 6% |
| Other | 3 | 10% |
| Unknown | 6 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 35% |
| Biochemistry, Genetics and Molecular Biology | 8 | 26% |
| Medicine and Dentistry | 3 | 10% |
| Business, Management and Accounting | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 6 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 October 2018.
All research outputs
#7,170,037
of 22,663,150 outputs
Outputs from AMB Express
#156
of 1,227 outputs
Outputs of similar age
#49,213
of 155,482 outputs
Outputs of similar age from AMB Express
#1
of 5 outputs
Altmetric has tracked 22,663,150 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,227 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 155,482 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them