| Title |
Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1
|
|---|---|
| Published in |
AMB Express, February 2012
|
| DOI | 10.1186/2191-0855-2-13 |
| Pubmed ID | |
| Authors |
Xu-Fen Zhu, Hai-Qin Tan, Chu Zhu, Li Liao, Xin-Qi Zhang, Min Wu |
| Abstract |
A chitosanase gene, csn, was cloned from Penicillium sp. D-1 by inverse PCR. The cDNA sequence analysis revealed that csn had no intron. The deduced CSN protein consists of 250 amino acids including a 20-amino acid signal peptide, and shared 83.6% identity with the family 75 chitosanase from Talaromyces stipitatus (B8M2R4). The mature protein was overexpressed in Escherichia coli and purified with the affinity chromatography of Ni2+-NTA. The novel recombinant chitosanase showed maximal catalytic activity at pH 4.0 and 48°C. Moreover, the activity of CSN was stable over a broad pH range of 3.0-8.0, and the enzymatic activity was significantly enhanced by Mg2+ and Mn2+. The CSN could effectively hydrolyze colloidal chitosan and chitosan, while could not hydrolyze chitin and carboxymethylcellulose (CMC). Due to the particular acidophily, CSN has the potential application in the recycling of chitosan wastes.The GenBank/EMBL/DDBJ accession numbers for the 18S rRNA gene and chitosanase gene of strain D-1 are JF950269 and JF950270, respectively. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 31% |
| Student > Master | 4 | 15% |
| Student > Doctoral Student | 1 | 4% |
| Student > Bachelor | 1 | 4% |
| Lecturer > Senior Lecturer | 1 | 4% |
| Other | 4 | 15% |
| Unknown | 7 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 46% |
| Biochemistry, Genetics and Molecular Biology | 4 | 15% |
| Unspecified | 1 | 4% |
| Engineering | 1 | 4% |
| Unknown | 8 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 February 2012.
All research outputs
#22,759,452
of 25,373,627 outputs
Outputs from AMB Express
#1,038
of 1,325 outputs
Outputs of similar age
#152,658
of 167,764 outputs
Outputs of similar age from AMB Express
#6
of 7 outputs
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