| Title |
Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus
|
|---|---|
| Published in |
AMB Express, March 2016
|
| DOI | 10.1186/s13568-016-0188-x |
| Pubmed ID | |
| Authors |
Geomárcia Feitosa da Cruz Ramos, Patricia Locosque Ramos, Michel Rodrigo Zambrano Passarini, Marghuel A. Vieira Silveira, Débora Noma Okamoto, Lilian Caroline Gonçalves de Oliveira, Larissa Vieira Zezzo, Alyne Marem, Rafael Costa Santos Rocha, João Batista da Cruz, Luiz Juliano, Suzan Pantaroto de Vasconcellos |
| Abstract |
The bioprospection for cellulase and protease producers is a promise strategy for the discovery of potential biocatalysts for use in hydrolysis of lignocellulosic materials as well as proteic residues. These enzymes can increment and turn viable the production of second generation ethanol from different and alternative sources. In this context, the goal of this study was the investigation of cellulolytic and proteolytic abilities of bacteria isolated from the gastrointestinal tract of a hippopotamus as well as from its composting process. It is important to highlight that hippopotamus gastrointestinal samples were a non-typical sources of efficient hydrolytic bacteria with potential for application in biotechnological industries, like biofuel production. Looking for this, a total of 159 bacteria were isolated, which were submitted to qualitative and quantitative enzymatic assays. Proteolytic analyzes were conducted through the evaluation of fluorescent probes. Qualitative assays for cellulolytic abilities revealed 70 positive hits. After quantitative analyzes, 44 % of these positive hits were selected, but five (5) strains showed cellulolytic activity up to 11,8 FPU/mL. Regarding to proteolytic activities, six (6) strains showed activity above 10 %, which overpassed results described in the literature. Molecular analyzes based on the identification of 16S rDNA, revealed that all the selected bacterial isolates were affiliated to Bacillus genus. In summary, these results strongly indicate that the isolated bacteria from a hippopotamus can be a potential source of interesting biocatalysts with cellulolytic and proteolytic activities, with relevance for industrial applications. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 29 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 17% |
| Student > Bachelor | 4 | 14% |
| Student > Ph. D. Student | 4 | 14% |
| Student > Doctoral Student | 3 | 10% |
| Other | 3 | 10% |
| Other | 5 | 17% |
| Unknown | 5 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 28% |
| Agricultural and Biological Sciences | 7 | 24% |
| Engineering | 3 | 10% |
| Environmental Science | 2 | 7% |
| Immunology and Microbiology | 2 | 7% |
| Other | 1 | 3% |
| Unknown | 6 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 March 2016.
All research outputs
#15,362,070
of 22,852,911 outputs
Outputs from AMB Express
#445
of 1,235 outputs
Outputs of similar age
#177,096
of 298,399 outputs
Outputs of similar age from AMB Express
#15
of 26 outputs
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