@mad_molix ご存じかと思いますがテープサンドイッチはこんな感じです。https://t.co/476wbyWWft ノンホモジェナイズドの牛乳ですか。思いもつかない材料でした。たくさん材料があるんですね。
@mdelucas80 @0_minyaaa We just follow https://t.co/K8sKr4530D and it works like a charm. PEG brand apparently makes a huge difference, this happens in other contexts with other MW PEGs as well. I can also share the bench protocol, just PM!
@ATinyGreenCell When I was doing Arabidopsis work, we used the 'Tape sandwich' method - much, much simpler. We just used regular electrical tape for the base and sticky tape for the top. Might it work for your system? https://t.co/7UqOOo9ckk
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
RT @jbrumos7: Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & om…
Protoplast transient expression systems are versatile tools to study gene function using cellular, genetic, biochemical, & omics approaches. This protocol improves isolation & transfection steps making it a workable assay for nearly any plant speci
@leonardojo @BraybrookSA That might be it. Try using more starting material, more leaves and shorten their time in the digestion buffer. I don't know if you can "peel" soybean leaves but you could try this protocol. It is fantastic for preparing Arabidopsi
Method paper here https://t.co/DG5I7kgs7f
@TCHTSYM 私も一回、葉の表皮をとるのに3Mのテープを探したことがあります。tape sandwich法とかいうみたいです。これのFig1 https://t.co/476wbyWWft
@taylorlabsoton if you want, you can check the methods paper we use as a guide here https://t.co/DG5I7jYQIF